硫酸酯化琼脂糖的抗凝血性能  

Anticoagulation properties of sulfated agarose

作　　者：揭油平[1] 尤玲玲[1] 梁婉爱[1] 汤顺清[1] 

机构地区：[1]暨南大学生物医学工程研究所,广东省教育厅生物材料重点实验室,广东省广州市510632

出　　处：《中国组织工程研究与临床康复》2010年第16期2907-2910,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家863项目(2007AA09Z436)~~

摘　　要：背景:多糖的硫酸酯化一直是近年来的研究热点,其中对于硫酸酯化多糖的抗凝血性能,近年来较多从取代度层面进行探讨,相对分子质量及空间结构方面的影响较为少见。目的:观察不同相对分子质量硫酸酯化琼脂糖的抗凝血性能。方法:以琼脂糖为原料,甲酰胺为分散剂,通过氯磺酸吡啶法制备了硫酸酯化琼脂糖。通过透析纯化分级,获得两种不同相对分子质量分布的琼脂糖硫酸酯。采用人体血浆的部分凝血活酶时间、凝血酶原时间指标分别对它们的抗凝血性能进行了研究。结果与结论:可以获得取代度及取代位置相似,具有不同相对分子质量分布的硫酸酯化琼脂糖。对凝血指标的化验结果证明,在一定范围内,相对分子质量大的产物的抗凝血活性大于相对分子质量小的产物。结果提示相对分子质量对硫酸酯化琼脂糖的抗凝血活性有重要的影响。BACKGROUND：Polysaccharide sulfate has been hot focus in recent research.Regarding anticoagulant property of sulfated polysaccharides,recent studies mainly explored degree of substitution,and there are rare studies concerning relative molecular mass and spatial structure.OBJECTIVE：To study the anticoagulant activity of the sulfated agarose with different relative molecular masses.METHODS：With formamide as dispersing agent,agarose was sulfated with the method of chlorosulfonic acid-pyridine.The product was graded and purified with dialysis bag.Two kinds of sulfated agarose with different molecular masses were obtained,and their anticoagulant properties were checked by assays of the activated partial thromboplastin time,thrombin time.RESULTS AND CONCLUSION：Two kinds of sulfated agarose had similar degree of substitution and sulfation position,whereas their molecular weight was different.The bioassay results of coagulation index demonstrated that the anticoagulant activity of agarose improved with the increasing of molecular mass within a certain range.Relative molecular mass had an important effect on the anticoagulant activity of the sulfated agarose.

关 键 词：硫酸酯化琼脂糖 相对分子质量 抗凝血 部分凝血活酶时间 凝血酶原时间 生物材料 

分 类 号：R318[医药卫生—生物医学工程]