PcDNA3.1-rhGM-CSF重组质粒的构建及鉴定  

Construction and Identification of Eukaryotic Expression Vector of PcDNA3.1-rhGM-CSF

作　　者：朱红[1] 唐恩洁[1] 杨晓红[1] 

机构地区：[1]川北医学院基础医学院微生物与免疫学教研室,四川南充637007

出　　处：《川北医学院学报》2010年第2期106-108,共3页Journal of North Sichuan Medical College

基　　金：四川省科技厅项目资助(编号:2009SZ0260)

摘　　要：目的:构建PcDNA3.1-rhGM-CSF真核细胞表达载体。方法:采用PCR、T-A克隆及基因定向克隆技术,将rhGM-CSF基因插入PcDNA3.1(+)空载体,构建了PcDNA3.1-rhGM-CSF真核表达载体。经限制性内切酶酶切和DNA测序进行鉴定。结果:双酶切及DNA测序证实PcDNA3.1-rhGM-CSF真核细胞表达载体构建成功。结论:成功构建PcDNA3.1-rhGM-CSF真核细胞表达载体。Objective：To construct PcDNA3.1-rhGM-CSF expression vector in order to make clear the role of rhGM-CSF in the differentiation of leukemic cell.Methods： The eukaryotic expression plasmid PcDNA3.1-hGM-CSF was constructed by means of PCR,T-A clone and directional cloning techniques.The combinants were finally sequenced and identified by restriction endonuclease digestion.Results： PcDNA3.1-rhGM-CSF expression vector was successfully constructed and identified by double endonuclease digestion and sequence analysis.Condusion： PcDNA3.1-rhGM-CSF expression vector has been successfully constructed.

关 键 词：粒细胞-巨噬细胞集落刺激因子 重组表达载体 构建 

分 类 号：R392.13[医药卫生—免疫学]