加替沙星印迹聚合物的制备及结合特性研究  

Study on the Preparation of Gatifloxacin Imprinted Polymers and its Binding Properties

作　　者：马晶[1] 杨明[1] 周魁[1] 盛鑫鑫[1] 

机构地区：[1]武汉工业学院化学与环境工程学院,武汉430023

出　　处：《化学通报》2010年第5期455-460,共6页Chemistry

摘　　要：以微米级聚苯乙烯微球为种子、加替沙星为模板分子,采用单步溶胀聚合法,在水相中制得了分子印迹聚合物微球(MIPMs)。通过紫外光谱研究MIPMs的识别机理;利用红外光谱分析MIPMs的结合位点,运用扫描电镜观察微球的形貌;并结合加替沙星的荧光性质,利用荧光光谱研究MIPMs的结合特性。Scatchard分析表明,MIPMs在识别加替沙星过程中存在两类结合位点,高亲和力结合位点的平衡离解常数KD1=0.603mmol/L,最大结合量Bmax1=28.62μmol/g;低亲和力结合位点的平衡离解常数KD2=3.110mmol/L,最大结合量Bmax2=74.27μmol/g。Molecular imprinted polymeric microspheres（MIPMs ） were prepared by a one-step swelling polymerization method in an aqueous system using polystyrene microspheres as seeds and Gatifloxacin as template molecules.The recognition mechanism of MIPMs was evaluated by ultraviolet spectrometry.The infrared spectrum of the polymer further demonstrated that there were some functional groups in the MIPMs which could interact with the template molecules.The morphology of the MIPMs was measured by scanning electron microscopy.The binding characteristics were investigated by fluorescence spectrum.Scatchard analysis suggested that there were two classes of binding sites during MIPMs recognition of Gatifloxacin.Equilibrium dissociation constant KD1 and maximum number Bmax1 of binding site with high affinity were calculated to be 0.603 mmol /L and 28.62 μmol /g,while KD2 and Bmax2 of binding site with low affinity were 3.110 mmol /L and 74.27 μmol /g,respectively.

关 键 词：分子印迹 加替沙星 结合位点 Scatchard分析 抗生素污染 

分 类 号：TQ465[化学工程—制药化工]