均匀设计法优选聚砜超滤膜分离姬松茸多糖工艺条件  

Study on optimum separate technique of Agarcus blazei Murill(ADM) polysaccharides with PS ultrafiltration membrane by uniform design method

作　　者：谢飞[1] 叶晓[1] 

机构地区：[1]北京联合大学生物化学工程学院,北京100023

出　　处：《食品科技》2010年第5期120-124,共5页Food Science and Technology

基　　金：北京市教委科研计划项目(KM200911417008)

摘　　要：利用均匀设计法优选确定了聚砜超滤膜分离姬松茸多糖的工艺条件。实验考察了多糖料液浓度、操作压力、料液温度3个因素对多糖通量和多糖截留率2个指标的影响,通过对实验数据的多元线性回归分析得到了可对多糖通量和截留率2个指标进行预测的回归方程。研究结果表明:(1)通量的回归方程Y1=10.1862-0.5815X1+45.7999X2+0.2184X3。多糖料液浓度X1、操作压力X2、料液温度X33个变量因子对通量均有显著影响,按照影响从大到小排序为:多糖料液浓度(77.22%)>操作压力(11.93%)>多糖温度(10.85%),其中多糖料液浓度对通量指标的贡献是负值,操作压力、多糖温度对通量指标的贡献是正值。(2)截留率的回归方程Y2=93.9005+0.0727X1-24.479X2-0.0641X3。多糖料液浓度X1、操作压力X2对截留率均有显著影响,料液温度X3对截留率也有影响。按照影响从大到小排序为:操作压力(61.42%)>多糖料液浓度(21.73%)>多糖温度(16.85%),其中操作压力、多糖温度对截留率指标的贡献是负值,多糖料液浓度对截留率指标的贡献是正值。Studies on optimum separate technique of Agarcus blazei Murill（ABM） polysaccharides with PS ultrafiltration membrane by uniform design method.By observing the effect of the three factors：ABM solution concentration,operation pressure,and solution temperature,on the two targets：flux and retention.Based on the liner multi-regression analysis of these data,we have been got two regression equationes which can forecast the two targets：flux and retention extraction.The results showed that：（1） The regression equation of flux：Y1= 10.1862-0.5815X1＋45.7999X2＋0.2184X3.For membrane flux,the concentration X1,pressure X2 and temperature X3 were all the obvious influence factors,and the pressure and temperature played a positive role in the flux,but the concentration played a negative contribution.The influence degrees of the three factors on the flux were orderly：concentration（77.22%）pressure（11.93%） temperature（10.85%）;（2）The regression equation of retention：Y2=93.9005＋0.0727X1-24.479X2-0.0641X3.For membrane retention,both the concentration X1 and pressure X2 were theremarkable influence factors and the temperature X3 was only an influence factors.The pressure and temperature played a negative role in the retention,but the concentration played a positive contribution.The influence degrees of the three factors on the retention were orderly：pressure（61.42%）concentration（21.73%） temperature（16.85%）.

关 键 词：均匀设计法 超滤膜分离 姬松茸多糖 多元线性回归 

分 类 号：Q028.8[生物学]