白细胞介素-10对支气管哮喘小鼠肺部炎症的影响  

Influence of Interleukin-10 on Pneumonic Anaphylacticitis in Mice with Asthma

作　　者：张磊[1] 曲书强[1] 张凤蕴[2] 

机构地区：[1]哈尔滨医科大学附属第二医院儿内科,哈尔滨150086 [2]哈尔滨医科大学免疫学教研室,哈尔滨150086

出　　处：《实用儿科临床杂志》2010年第9期634-636,共3页Journal of Applied Clinical Pediatrics

基　　金：黑龙江省教育厅资助项目(10531106)

摘　　要：目的探讨IL-10对支气管哮喘(哮喘)小鼠肺部变应性炎症的抑制作用及对血清IgE水平的影响。方法 30只6～8周龄清洁级雄性BALB/c小鼠,体质量(20±2)g,随机分为正常组,哮喘组和IL-10组,每组各10只。哮喘组和IL-10组以1g.L-1卵白蛋白(VOA)腹腔注射致敏,以10g.L-1VOA20mL雾化吸入激发制作小鼠哮喘模型。正常组不做任何处理。哮喘组每次激发前予9g.L-1盐水0.1mL鼻内滴入,IL-10组每次激发前给予含IL-10(0.2μg.L-1)的9g.L-1盐水0.1mL鼻内滴入。末次激发24h后,经小鼠眼球采血分离血清,采用ELISA法测定其血清IgE水平。取其肺组织行病理切片。结果哮喘组小鼠病理改变与其他各组有明显区别,支气管和血管周围炎性细胞浸润(主要为嗜酸性粒细胞,中性粒细胞),黏膜及黏膜下层水肿明显,呼吸道中可见较多的黏液和黏液栓。而IL-10组与正常组无明显区别:肺泡结构清晰,肺泡腔和支气管腔无渗出物,与哮喘组比较有显著性差异。正常组、哮喘组和IL-10组小鼠血清IgE水平分别为(5.219±0.427)μg.L-1、(6.633±0.145)μg.L-1和(5.503±0.566)μg.L-1,哮喘组血清IgE水平与正常组及IL-10组比较差异均有统计学意义(Pa<0.05),IL-10组与正常组比较差异无统计学意义(P>0.05)。结论 IL-10局部滴入治疗可抑制哮喘小鼠肺部变应性炎症反应,改善其呼吸道症状,降低哮喘小鼠血清IgE水平。Objective To explore the inhibition effect of interleukin - 10 （ IL - 10） on pneumonic anaphylacticitis in mice with asthma and its effects on the level of serum IgE. Methods Thirty BALB/c male mice whose weight were （20 ± 2） g were randomly divided into 3 groups ：normal group （n = 10）, asthma model group （n = 10 ） and IL - 10 treatment group （n = 10）. Asthma model group and IL - 10 group were sensifizod with 1 g· L^-1 ovalbumin,then asthma model was made with inhallating 10 g · L^-l VOA 20 mL. The normal group was not administered anything as a negative group. Before inhallating with 10 g · L^- VOA, asthma model group was administered inhalation with 9 g· L^-1 saline 0.1 mL, and IL - 10 treatment group was administered inhalation with 0.1 mL IL - 10 whose concentration was 0.2 ug·L^-1. The level of IgE was measured by ELISA reagent box, eviscerated lungs after collecting blood and then made them into pathological slices. Results There was/an obvious distinction in the mice pathological change of lungs and bronchus between asthmamodel group and other groups, but no significan difference between IL - 10 treatment group and normal group. There was no obvious infiltration of inflammatory cells in the airways of IL =- 10 treatment group and normal group. However, there were a great number of inflammatory cells in the interstitial and peribron- chovaseular regions of asthma model group. The levels of serum IgE were （5. 219±0.427） ug·L^-1 , （6.633 ±0. 145）ug·L^-1 and （5.503 ± 0.566）ug·L^-1 in normal group, asthma model group and IL- 10 treatment group, respectively. The level of serum lgE in asthma model group increased obviously compared with normal group and IL - 10 treatment group, with significant differences （P. 〈 0.05 ）. There was no significant difference between IL - 10 treatment group and normal group （ P 〉 0. 05 ）. Conduslons Inhalation of IL - 10 can optimally control the pneurnonic anaphylacticitis, improve the situation of airway inflammation in mi

关 键 词：哮喘 白细胞介素-10 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统]