支气管哮喘患儿T淋巴细胞亚群变化及相关细胞因子对Th细胞的调节  

Changes of T Cell Subsets and Regulation of Cytokine on Helper T Cell in Asthmatic Children

作　　者：陈杰[1,2] 袁岳沙[1,3] 李琦[4] 刘梅[3] 靳伟 周少华[3] 

机构地区：[1]长江大学临床医学院儿科,湖北荆州434000 [2]黄石第二人民医院呼吸内科,湖北黄石435002 [3]长江大学附属荆州第一人民医院儿科,湖北荆州434000 [4]长江大学附属荆州第一人民医院临床免疫室,湖北荆州434000

出　　处：《实用儿科临床杂志》2010年第9期643-645,共3页Journal of Applied Clinical Pediatrics

基　　金：湖北省教育厅自然科学研究重点基金项目(2004X055)

摘　　要：目的观察支气管哮喘患儿急性发作期T淋巴细胞亚群水平变化及IL-27对Th细胞的调节作用,探讨其与哮喘发病的关系。方法选择10例哮喘患儿(哮喘组)和5例健康儿童(健康对照组)为研究对象。流式细胞仪检测其外周静脉血T淋巴细胞亚群(CD4+T、CD8+T、CD4+T/CD8+T);收集其外周血单个核细胞(PBMC),加入不同水平(5.0μg.L-1和0.5μg.L-1)重组人IL-27(rhIL-27)进行体外培养12h,ELISA法测定其培养上清液中IFN-γ、IL-4水平。结果哮喘组CD4+T淋巴细胞,CD4+/CD8+[(38.408±3.808)%,1.480±0.155]均显著高于健康对照组[(28.240±1.936)%,1.151±0.092](Pa<0.05),CD3+[(60.354±4.770)%]、CD8+T淋巴细胞[(26.080±2.592)%]与健康对照组[(61.978±4.020)%,(24.706±2.753)%]比较差异均无统计学意义(Pa>0.05);不同水平的rhIL-27刺激PBMC进行体外培养后,IFN-γ表达[(116.160±54.890)μg.L-1,(9.369±6.910)μg.L-1]较健康对照组[(7.844±8.380)μg.L-1]均显著升高(Pa<0.005);高水平rhIL-27(5.0μg.L-1)组IL-4水平[(12.600±2.433)μg.L-1]较健康对照组[(70.820±22.912)μg.L-1]明显降低(P<0.01),而低水平rhIL-27(0.5μg.L-1)组IL-4水平[(81.480±6.396)μg.L-1]与健康对照组[(70.820±22.912)μg.L-1]比较差异无统计学意义(P>0.05)。结论 CD4+T淋巴细胞(Th细胞)在哮喘的炎症反应中起重要作用,且IL-27可调控Th1/Th2细胞,为哮喘治疗提供一种新的途径。Objective To investigate the changes of T lymphocyte subsets in asthmatic children and effect of IL - 27 on cytokine production of helper T lymphocyte subsets （Thl/Th2）. Methods The subsets of T lymphocyte in 10 cases of asthmatic children （ asthmatic group） in attacking stage and 5 healthy children （healthy control group）were measured by flow cytometry. PBMCs from 10 asthmatic children were cultured with recombinant human IL -27 （5 ug ~ L^-1 or 0. 5 ug ~ L^-1 ） for 12 h in vitro, and then the levels of IFN and IL -4 in their supernatant were measured by ELISA. Results The levels of CD4 ＋ T cell [ （38. 408 ± 3. 808） % ] and ratio of CD4＋/CD8＋ T cell （ 1. 480 ± 0. 155 ） in asthmatic children were significantly increased than those in healthy control group [ （28. 240 ± 1. 936） % , 1.151 ± 0. 092 ] （ P 〈 0.05 ） , but the levels of CD3 ＋ T cell[ （60. 354 ± 4. 770） % ] and CD8 ＋ T cell[ （26. 080 ± 2. 592） % ] in asthmatic group had no remarkable difference compared with healthy control group[ （61. 978 ± 4. 020）% , （24. 706 ±2. 753 ）% ] （P 〉 0.05 ）. PBMCs from children were divided into 3 groups ,5.0ug·L^-1 IL-27 group, 0. 5ug·L^-1 IL-27 group and the healthy control group. The values of IFN - γ, in supernatant were （116.160±54.89）ug·L^-1,（9.369±6.910） ug·L^-1 and （7.844±8.380） ug·L^-1, respectively. The levels of IFN-γafter stimulation with IL - 27 were remarkably higher than those in healthy control group （ P〈 0. 005 ）. The levels of IL-4 in 5.0ug·L^-1 IL - 27 group ,0.5 ug·L^-1 IL- 27 group and healthy control group were （12. 600 ± 2. 433 ）ug·L^-1, （81. 480±6. 396） ug·L^-1 and （70. 820 ± 22.912）ug·L^-1 ,respectively. The levels of IL-4 in 5 ug·L^-1 IL-27 group had a significant decrease compared with that in healthy control group（P 〈0.01 ） ,but the value of IL-4 in 0.5 ug·L^-1 IL- 27 group had no significant difference compared with that in healthy control group （ P 〉 0.05 ）. Conclu

关 键 词：哮喘 T淋巴细胞 白介素27 儿童 

分 类 号：R725.6[医药卫生—儿科]