复方大黄素组合体外抗疱疹病毒Ⅰ型的作用  

Study on the inhibitory action on Herpesvirus-Ⅰreplication in vitro by the compatibility of emodin and other medicine components

作　　者：雷湘[1,2] 陈科力[3] 陈刚[3] 杨占秋[1] 

机构地区：[1]武汉大学基础医学院病毒研究所国家病毒学重点实验室,湖北武汉430071 [2]武汉科技大学中南分校生命科学院,湖北武汉430223 [3]湖北中医学院药学院,湖北武汉430064

出　　处：《中国医院药学杂志》2010年第9期770-774,共5页Chinese Journal of Hospital Pharmacy

基　　金：湖北省自然科学基金资助(编号:2009CDZ014)

摘　　要：目的:研究大黄素与不同中药活性成分配伍后体外抗疱疹病毒Ⅰ(HSV-Ⅰ)的作用,并对其抗病毒机制进行初步探讨。方法:用不同组成、不同浓度的中药溶液处理HSV-Ⅰ感染的Vero细胞,72h后四甲基偶氮唑盐(MTT)法测定细胞活性并用空斑减数法进行验证。结果:在Vero细胞系中,所有经配伍后的中药组合都有一定的抑制HSV-Ⅰ的细胞病变效应(CPE)的作用,使细胞存活率提高,同时其细胞毒性也有所降低。结论:中药配伍大黄素后,可以起到减毒增效的作用。OBJECTIVE To investigate the anti-herpesvirus-I activity of the compatibility of emodin and other medicine component in Vero cells, and to explore their mechanism of antiviral action. METHODS Vero cells infected by HSV- I were cultured with different concentration and proportion of the emodin and other medicine components for 72 hours and their inhibitory effects on HSV- I replication were evaluated with cell survival rates by MTT assay and Lacunae Subtrahend assay. RESULTS In the Vero cell system, all the medicine components could inhibit cytopathic effect（CPE） of HSV-infected cells, and increase cells survival rates,the toxicity of emodin was reduced. CONCLUSION The toxicity of emodin was reduced and its anti- HSV- I activity was enhanced by the compatibility of other medicine components.

关 键 词：大黄素 疱疹病毒酒己伍 

分 类 号：R282[医药卫生—中药学]