农杆菌介导SAD基因转化浅白隐球酵母的条件优化  

Conditions Optimization of Transforming Cryptococcus albidus via Agrobacterium tumefaciens Mediate SAD Gene

作　　者：陈东亮[1] 李纪元[1] 范正琪[1] 范妙华[1] 田敏[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《湖南农业科学》2010年第4期16-19,共4页Hunan Agricultural Sciences

基　　金：浙江省重大科技攻关项目(2006C120301)

摘　　要：为使产油真菌浅白隐球酵母的油脂更适合生物柴油的要求,利用根癌农杆菌介导的遗传转化方法,将植物SAD基因转入浅白隐球酵母中,并对影响转化效率的关键因子进行了优化。结果表明:利用农杆菌菌株AGL-1介导,农杆菌浓度为1×109个/mL,酵母浓度为酵母密度为1×107～2×107个/mL,在含有AS200μmol/L条件下共培养48h可获得较高的转化率。PCR检测表明,SAD基因已成功转入浅白隐球酵母中,证明了该转化系统对浅白隐球酵母遗传转化的可行性。Agrobacterium tumefaciens-mediated transformation method was used to transform plants SAD gene into Cryptococcus albidus.Key factors affecting transformation were optimized.The results showed that when cultured sample for 48 h under the conditions that mediated by Agrobacterium tumefaciens strain（AGL-1）,concentration of Agrobacterium tumefaciens was 1×109 strains/mL,concentration of Cryptococcus albidus was 1×107～2×107 strains/mL and concentration of AS was 200 μmol/L,the transformation rate was higher.PCR detection determined that SAD gene has been transformed into Cryptococcus albidus.All the results above proved that the ATMT system is feasible to genetic transformation of Cryptococcus albidus.

关 键 词：根癌农杆菌 SAD 遗传转化 浅白隐球酵母 

分 类 号：Q933[生物学—微生物学]