PCR检测诊断罗非鱼荧光假单胞菌病技术的建立  

Development of Detecting Pseudcmonas fluoroscercs in Tilapia by PCR

作　　者：邓显文[1] 谢芝勋[1] 刘加波[1] 谢志勤[1] 谢丽基[1] 庞耀珊[1] 

机构地区：[1]广西兽医研究所,广西南宁530001

出　　处：《湖南农业科学》2010年第4期126-128,共3页Hunan Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0428001-2)

摘　　要：根据基因库荧光假单胞菌的16S-23S rDNA基因序列设计一对特异性引物,用PCR对4株鱼荧光假单胞菌进行扩增。特异性结果显示该对引物对4株荧光假单胞菌均扩增出与预期大小相一致的428bp的扩增产物,而对罗非鱼嗜水气单胞菌、温和气单胞菌、罗非鱼迟缓爱德华氏菌、斑点叉尾鮰钻鱼爱德华氏菌、柱状嗜纤维菌、链球菌、葡萄球菌、河弧菌、大肠杆菌和沙门氏菌等10种病原体的扩增,结果全为阴性。该PCR敏感性结果表明可以检测到1pg的荧光假单胞菌DNA模板。A pair of primers were designed and synthesized according to the published gene sequence（16S-23S rDNA） of Pseudcmonas fluoroscercs.The DNAs of four Pseudcmonas fluoroscercs strains,isolated from Tilapia,were amplified by the polymerase chain reaction（PCR） method,and 428 bp long amplified products were obtained consistent with anticipation.But the amplification of this pair of primers on other 10 pathogens（Aeromonas hydrophila,Aeromonas sobria,Edwardsiella tarda,Edwardsiella ictarda,Cytophaga columnaris,Streptococcus,staphylococci,vibrio,E.coli and salmonella） failed to show any positive detection.The sensitivity results revealed that 1 pg of Pseudcmonas fluoroscercs DNA were able to be detected by this PCR.

关 键 词：罗非鱼 荧光假单胞菌 PCR 检测. 

分 类 号：S941.1[农业科学—水产养殖]