脑脉通有效部位中总皂苷的含量测定  

Content Determination of Total Saponins in Effective Composition of Naomaitong

作　　者：罗兰[1] 崔永霞[2] 王淑美[1] 高淑娟[2] 陈国芹[2] 李建生[2] 

机构地区：[1]广东药学院,广州市510006 [2]河南中医学院,郑州市450008

出　　处：《中国药房》2010年第19期1761-1763,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30371812)

摘　　要：目的:建立脑脉通有效部位中总皂苷的含量测定方法。方法:采用比色法,以人参二醇为对照品,在556nm波长处测定吸光度。结果:人参二醇的检测浓度在2.03～10.15μg·mL-1范围内与吸光度值呈良好线性关系(r=0.9996);平均回收率为100.69%,RSD=1.76%(n=6)。结论:本方法操作简便、准确、灵敏、重复性好,可用于脑脉通的质量控制。OBJECTIVE：To establish the method for the content determination of total saponins in Naomaitong.METHODS：Colormetric method was applied using panoxadiol as control.The detection wavelength was set at 556 nm.RESULTS：The linear range of panoxadiol was 2.03～10.15 μg·mL^-1（r=0.999 6） with an average recovery of 100.69%（RSD=1.76%,n=6）.CONCLUSION：The method is easy to operate with high precision,accuracy and good reproducibility for the quality control of Naomaitong.

关 键 词：脑脉通 总皂苷 含量测定 比色法 

分 类 号：R283[医药卫生—中药学] R927.2[医药卫生—中医学]