高效毛细管电泳法测定新疆紫草中左旋紫草素的含量  

Content Determination of Shikonin in Arnebia euchroma by HPCE

作　　者：白研[1] 毋福海[1] 罗碧莲[1] 曾春丽[1] 

机构地区：[1]广东药学院公共卫生学院,广州市510310

出　　处：《中国药房》2010年第19期1790-1792,共3页China Pharmacy

摘　　要：目的:建立以高效毛细管电泳-紫外检测法测定新疆紫草中左旋紫草素含量的方法。方法:分离柱为未涂层弹性融硅石英毛细管(53cm×50μm ID,有效长度46cm),电泳介质为2.0mmo·lL-1H3BO3+6.0mmo·lL-1三乙胺缓冲液+5.0mmo·lL-1β-环糊精,分离电压为14kV,重力进样时间15(s高度25cm),检测波长为516nm。结果:左旋紫草素的检测浓度在7.2～36.0μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9990);平均回收率为100.15%,RSD=1.68%(n=6)。结论:本法简便、快速、准确、可靠,可用于新疆紫草中左旋紫草素的含量测定。OBJECTIVE：To determine the content of shikonin in Arnebia euchroma by HPCE-UV.METHODS：The separation was performed on uncoated fused silica capillary column （53 cm×50 μm ID,46 cm effective length）.2.0 mmol·L^-1 H3BO3,6.0 mmol·L^-1 triethylamine buffer solution and 5.0 mmol·L^-1β-CD was used as background electrolyte,14 kV separation voltage and time of gravity injection 15 s （25 cm）.The detection wavelength was set at 516 nm.RESULTS：The linear range of shikonin was 7.2～36.0 μg·mL^-1（r=0.999 0） with an average recovery of 100.15%（RSD=1.68%,n=6）.CONCLUSION：The method is convenient,rapid,accurate and reliable for the content determination of shikonin in A.euchroma.

关 键 词：高效毛细管电泳法 新疆紫草 左旋紫草素 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]