脑死亡供体吸入一氧化碳对肺移植后细胞凋亡的影响  

Effect of carbon monoxide inhalation on apoptosis of lung graft from brain death donor in rats

作　　者：金笛[1] 周华成[1] 李文志[1] 潘鹏[1] 刘金锋[1] 丁文刚[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院麻醉科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第2期103-106,共4页Journal of Harbin Medical University

基　　金：国家自然科学基金(30571784)

摘　　要：目的观察脑死亡(brain death,BD)肺移植供体吸入一氧化碳(carbon monoxide,CO)对移植肺细胞凋亡的影响。方法将24只大鼠随机分为3组,对照组(sham组)供体鼠颅内置入Fogarty导管,但不膨胀前端球囊;脑死亡组(BD组)和脑死亡CO治疗组(BDCO组)供体鼠颅内置入Fogarty导管,并膨胀前端球囊建立BD模型。sham组和BD组吸入氧气浓度为40%的氧氮混合气150 min,BDCO组在开始BD诱导后30 min,确认BD后,吸入氧气浓度为40%的氧氮混合气和250 ppm的CO2 h。然后进行肺移植,移植后2 h取移植肺组织切片进行TUNEL染色观察移植肺中细胞凋亡情况,使用RT-PCR和免疫组化技术观察Caspase-3 mRNA和蛋白表达情况。使用免疫组化评分(immunohistoche micas scores,IHS)对细胞凋亡和Caspase-3蛋白表达进行评估。结果BD组细胞凋亡程度,Caspase-3蛋白和mRNA表达显著高于sham组(P<0.05),BDCO组与BD组相比,上述指标显著降低(P<0.05)。结论供体BD过程增加了移植肺细胞凋亡比率,BD供体吸入CO对移植肺再灌注后细胞凋亡有拮抗效应。Objective To investigate whether carbon monoxide（CO） inhalation in brain death（BD） donor would show anti-apoptosis effects on lung grafts in rats.Methods Wistar rats were randomly divided into 3 groups（n=8）.In sham group,donor rats received insertion of a balloon catheter into the cranial cavity,but were not induced to BD,and were ventilated with 40% oxygen.In BD group,donor rats inhaled 40% oxygen 2 h after BD confirmation.In BDCO group,donor rats inhaled 250 ppm CO and 40% oxygen 2 h after BD confirmation.The left lungs of donor rat were harvested and orthotopic lung transplantation was performed.Recipient rats were sacrificed 2 h after lung transplantation by exsanguinations through right femur artery.TUNEL staining demonstrated the apoptosis in lung grafts.The expression of Caspase-3 mRNA and protein were detected by RT-PCR and immunohistochemistry.The expression of Caspase-3 protein was evaluated by immunohistoche mical scores（IHS）.Results The apoptosis and the expression of Caspase-3 mRNA and protein in BD group were higher than sham group（P〈0.05）.But in BDCO group,the apoptosis and the expression of Caspase-3 mRNA and protein were decreased significantly compared with BD group（P〈0.05）.Conclusion BD process in donor increases the ratio of apoptosis in lung grafts,and CO inhalation in BD donor exerts anti-apoptosis effects on lung grafts.

关 键 词：脑死亡 肺移植 一氧化碳 凋亡 

分 类 号：R655.3[医药卫生—外科学]