组织工程骨促进犬牙槽骨再生的体内研究  

Tissue Engineered Bone to Enhance Periodontal Regeneration:an Experimental Study in Dogs

作　　者：谭丽思[1] 林晓萍[1] 刘尧[2] 魏巍[1] 

机构地区：[1]中国医科大学附属盛京医院口腔科,辽宁沈阳110004 [2]中国医科大学口腔医学院

出　　处：《口腔医学研究》2010年第2期183-186,共4页Journal of Oral Science Research

基　　金：辽宁省教育厅科研基金(编号:202013124);辽宁省自然科学基金(编号:20042062)

摘　　要：目的:探讨以犬骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)为种子细胞,Bio-Oss小牛无机骨粉为支架,构建组织工程化骨,结合Bio-Gide胶原膜促进犬牙槽骨再生的可行性。方法:在3只犬的口腔里人工制作12个三壁骨缺损,每只犬的左侧为实验组,右侧为对照组。实验组:植入组织工程化骨;对照组:单纯植入Bio-Oss骨粉。手术后即刻及术后4周,8周,通过影像学和组织学方法(HE,Masson染色)检测骨缺损的再生效果。结果:术后8周,实验组X-ray可见缺损区新生骨骨量明显增加,切片HE染色见新生牙槽骨已充满骨缺损处,骨小梁成团状,Masson染色为红色;对照组X-ray可见骨缺损区阴影变浅,HE染色骨小梁排列凌乱,Masson染色为蓝绿色。结论:组织工程化骨促进犬牙槽骨再生是可行的。Objective： To study the feasibility of using the tissue engineered bone which was constructed with canine marrow mesenchymal stem cells（BMSCs） as seed cells and the Bio-Oss calf inogenic bone grains as scaffold materials combined with Bio-Gide collagen membrane to regenerate experimental horizontal alveolar bone.Methods： We made twelve bone defects in the oral cavity of 3 dogs artificially.The left was the experimental group and the right was the control group.Experimental group was implanted tissue engineered bone.Control group was merely implanted Bio-Oss.Imageology examination was performed at postoperative moment and postoperative 4 and 8 weeks respectively and histology examination was used in the end to evaluate the effect of the regeneration of the alveolar bone by both HE and Masson staining.Results： The results showed that the new bone mass of experimental group increased obviously by X-ray at the eighth week.The defect districts were filled with new alveolar bone.The bone trabeculae was small,multi-space and showed floccus shape with HE staining and the collagen was stained red by Masson staining.The shade of control group was lighter by X-ray at the eighth week.The defect districts were not full of new alveolar bone,and the bone trabeculae was in disorder at control group whose collagen was dyed blue-green by Masson staining.Conclusion： It is feasible to use tissue engineered bone to promote the generation of canine alveolar bone.

关 键 词：骨髓间充质干细胞 组织工程化骨 再生 Bio-Oss小牛无机骨粉 

分 类 号：R318[医药卫生—生物医学工程]