经皮穿刺兔腰椎椎体内注射平阳霉素对腰椎软骨终板及椎间盘的影响  

Effects of Percutaneously Puncturing Vertebrate Adjacent to Cartilage Endplate and Injecting Pingyangmycin on Lumbar Intervertebral Disc and Cartilage Endplate in Rabbits

作　　者：侯昌龙[1] 杨建勇[1] 庄文权[1] 范惠双[1] 谭国胜[1] 郭文波[1] 陈伟[1] 张中伟[1] 毛丽娟[1] 

机构地区：[1]中山大学附属第一医院介入放射科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2010年第2期221-224,237,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(项目编号:2008B030301049);NSFC-广东联合基金重点项目(U0732001)

摘　　要：【目的】评价经皮穿刺兔腰椎终板椎体侧,注射平阳霉素对腰椎终板及椎间盘的影响。【方法】选取36只新西兰大白兔,每兔设第5腰椎(L5)为实验组、第4腰椎(L4)为对照组。穿刺腰椎终板椎体侧,L5注射平阳霉素(2mg/mL)1mL,L4注射生理盐水1mL。术后第1、2、3、4、5周及3月随机选取6只行MRI检查,并取病理送检。测量对比第4周实验组MRI与病理切片的椎体病变面积。【结果】对照组MRI和病理学检查均无特异性变化,实验组MRI及病理第1～2周变化不明显,第3周MRI出现FST1WI低信号,T2WI及FST2WI呈稍高信号,第4周信号变化更明显;第3～4周病理终板椎体侧骨结构紊乱,软骨终板细胞减少、结构紊乱,纤维环及髓核无明显变化;第5周出现软骨终板和椎间盘退变表现,第3月退变表现更加明显。实验组第4周MRI与病理切片病变区面积有明显正相关性(r=0.965,P<0.001)。【结论】采用经皮穿刺兔腰椎终板椎体侧注射平阳霉素的方法可诱发腰椎终板及椎间盘退变。【Objective】 This study was designed to evaluate the effects of percutaneously puncturing vertebrate adjacent to cartilage endplate and injecting pingyangmycin on lumbar intervertebral disc and cartilage endplate in New Zealand Rabbits. 【Methods】 Thirty-six New Zealand white rabbits were enrolled in this study. The fifth lumbar vertebrate （L5） was injected with pingyangmycin as experimental group, and the fourth lumbar vertebrate （L4） injected normal sodium as control group. Six rabbits were selected randomly, then MRI and histological observation was performed in the first, second, third, fourth, fifth week and third month after operation respectively. Moreover, the correlation analysis was performed between MRI and histological measurements for areas of the lesion in L5. 【Results】 There was no obvious changes on MRI and histological examination in control group. For experimental group, there were also no obvious changes in the first two weeks after operation. However, in the third week, it demonstrated slightly hyperintense signal on T2WI and fat-suppression T2WI （FS T2WI）, while FS T1WI was hypointense signal. The signal changed more obviously in the fourth week. Histologically, the structure of vertibrates arranged disorderly, chondrocyte of endplate decreased and architecture became disorder. Anulus fibrosus and nucleus pulposus did not change. The cartilage endplate and intervertebral disc degenerated in the fifth week. Both of them degenerated more obviously in third month. There was a strong correlation between MRI and histological measurements for areas of the lesion in the fourth week （r=0.965, P〈0.001）. 【Conclusion】 Degeneration of lumbar intervertebral disc and cartilage endplate in New Zealand Rabbits can be induced by percutaneously puncturing vertebrate adjacent to cartilage endplate and injecting pingyangmycin.

关 键 词：软骨终板 椎间盘 退行性变 磁共振成像 组织学 

分 类 号：R634[医药卫生—外科学]