小鼠神经干细胞免疫原性的研究  

The immunogenicity of mouse neural stem cells

作　　者：高峰[1] 杨小峰[1] 郑学胜[1] 沈罡[1] 刘伟国[1] 

机构地区：[1]浙江大学医学院附属第二医院神经外科,浙江杭州310009

出　　处：《细胞与分子免疫学杂志》2010年第4期307-309,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：浙江省自然科学基金资助项目(Y205180)

摘　　要：目的:探讨小鼠神经干细胞(NSCs)免疫原性,以利于解决神经干细胞移植中的免疫排斥问题。方法:(1)首先将30只C57BL/6近交系小鼠随机分成两组,以BALB/c近交系小鼠的NSCs及肝细胞(作为体细胞对照组)分别进行腹腔主动免疫,然后将NSCs及肝细胞分别与各自免疫后小鼠的T淋巴细胞进行单向混合淋巴细胞培养,通过液闪计数仪检测各自T淋巴细胞增殖程度,从而比较两者免疫原性的强弱。(2)利用异硫氰酸荧光素标记抗体(FITC)及Phycoery-thrin(PE)抗体分别标记BALB/c小鼠NSCs主要组织相容性抗原复合物Ⅰ、Ⅱ(MHC-Ⅰ、MHC-Ⅱ),然后通过流式细胞术(FCM)分别检测BALB/c小鼠NSCs及其肝细胞的MHC-Ⅰ和MHC-Ⅱ阳性细胞比例,从而推断小鼠NSCs的免疫原性。结果:(1)NSC组液闪计数仪检测所得cpm值为16592.8±2865.3,肝细胞组为27815.0±2416.3,NSC组明显低于肝细胞组,差别有统计学意义(P<0.01)。(2)小鼠NSCs实验组中,约(87.55±3.65)%的增殖期神经干细胞没有检测到MHC-Ⅰ和MHC-Ⅱ分子表达;而作为对照组的小鼠肝细胞组中,仅有约(27.45±1.86)%的肝细胞未表达MHC-Ⅰ和MHC-Ⅱ分子。且NSC组MHC-Ⅰ和MHC-Ⅱ阴性细胞比例明显高于肝细胞组,差别有统计学意义(P<0.01)。结论:小鼠神经干细胞具有较弱的免疫原性,且其免疫原性明显低于同一个体的肝细胞。AIM：To explore the immunogenicity of mouse neural stem cells（NSCs）,thus help to solve the problem of immunologic rejection in NSCs transplantation. METHODS：（1） Thirty C57BL/6 mice were assigned randomly into two groups,then the two groups were immuned by intraperitoneal injection respectively with BALB/c mice＇s NSCs or hepatic cells （as control）. Afterwards one-way mixed lymphocyte culture was performed with the BALB/c mice＇s NSCs or hepatic cells and immuned C57BL/6 mice＇s T lymphocytes. Immune response of T lymphocyte was detected by liquid scintillation counter. （2）The MHC-Ⅰand MHC-Ⅱmolecules of BALB/c mice NSCs were stained with FITC-and PE-labeled antibody respectively. Then the expression of MHC-Ⅰand MHC-Ⅱ were deteced by flow cytometry. RESULTS：As shown by liquid scintillation counter,the cpm value of NSCs was 16592.8±2865.3,and that of the hepatic cells was 27815.0±2416.3（P〈0.01）. About （87.55±3.65）% NSCs expressed neither MHC-Ⅰnor MHC-Ⅱ,but there were only about （27.45±1.86）% hepatocytes showed both MHC-Ⅰand MHC-Ⅱnegative（P〈0.01）. CONCLUSION：The mouse NSCs show weaker immunogenicity and their immunogenicity is significantly weaker than their hepatocytes.

关 键 词：神经干细胞 免疫原性 混合细胞培养 主要组织相容性抗原 流式细胞术 

分 类 号：R651[医药卫生—外科学]