应用VEGF抗体预防术后腹腔粘连的实验研究  

Prevention of postoperative abdominal adhesions by an antibody to VEGF in mice

作　　者：郑振华[1] 张桦[1] 潘玉先[1] 高毅[1] 杨继震[1] 

机构地区：[1]第一军医大学附属珠江医院

出　　处：《世界华人消化杂志》1999年第3期227-229,共3页World Chinese Journal of Digestology

摘　　要：目的评价ＶＥＧＦ抗体预防小鼠术后腹腔粘连的能力．方法以昆明小鼠为动物模型，经过同一标准的腹膜损伤手术后，２４只昆明小鼠随机分成两组（每组１２只）：ＶＥＧＦ抗体治疗组，１５４ｍｍｏｌ／Ｌ氯化钠溶液对照组，分别在关腹前腹腔内灌注ＶＥＧＦ抗体及１５４ｍｍｏｌ／Ｌ氯化钠溶液．术后２０ｄ将小鼠处死，观察腹腔内粘连的情况，通过对粘连的程度、位置以及粘连类型的综合评价得出粘连分数（三级分类法）．结果ＶＥＧＦ抗体治疗组的平均粘连分数（１３６±０６７，中位数１０）明显低于对照组（２２５±０７５，中位数２０）（Ｐ＜００１），２～３级粘连发生率（２７３％）比对照组（８３３％）亦有明显减低（Ｐ＜００１）；无一例小鼠出现腹壁切口疝．AIM To assess the ability of vascular endothelial growth factor (VEGF) antibody in preventing postoperative abdominal adhesion formation in mice. METHODS Twenty four Kunming mice were randomly divided into two groups: anti VEGF group ( n =12) and control group ( n =12). After an identical peritoneal injury operation, mice were treated intraperitoneally with either VEGF antibody or 154mmol/L sodium chloride at the time of abdominal closure. The mice were killed on postoperative day 20 and the abdominal incisions and the development of intraabdominal adhesions were investigated. Adhesion scoring was based on an overall assessment of the extent, location, and type of adhesions present. RESULTS Anti VEGF group had significantly lower adhesion scores (1 36±0 67, M 1 0) than the control group (2 25±0 75, M 2 0). When two groups were analyzed for the presence of grade 2-3 adhesions (advanced significant adhesions), the anti VEGF group had a significantly lower incidence (27 3%) than the control group (83 3%). No incisional hernia was found in abdominal wall in any group. CONCLUSION The intraperitoneal administration of an antibody to VEGF could inhibit postoperative adhesion formation.

关 键 词：血管内皮 生长因子 抗体 腹腔粘连 术后 预防 

分 类 号：R656.01[医药卫生—外科学]