乳酸乳球菌作为基因工程受体菌研究进展  

Research Progresses on Lactococcus lactis as Cell Factory for Recombinant Protein Expression

作　　者：李芳[1,2] 李永明[2] 徐子伟[1,2] 张磊[1,2] 

机构地区：[1]浙江师范大学,金华321000 [2]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《生物技术通报》2010年第5期61-64,81,共5页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(30871799);浙江省重大科技专项(2008C02003-2)

摘　　要：乳酸乳球菌(Lactococcus lactis)是一种"公认安全"的革兰氏阳性细菌,广泛存在于人、畜的肠道中并发挥许多重要的生理功能。由于它兼具安全性与益生性,近几年来研究者们开始关注用乳酸乳球菌作为受体菌来表达外源蛋白。随着生物技术的发展,人们对乳酸乳球菌基因表达及调控过程的认识不断深入并构建了一系列表达,成功地表达了许多外源蛋白,初步展示出良好的应用前景。主要对近年来国内外将乳酸乳球菌作为外源蛋白表达受体菌方面的研究进展做简要综述。Lactoeoccus lactis as a Gram positive bacterium which play important functions in the gastrointestinal tract of animal or human have been classified as ＂generally regarded as safe＂ （GRAS） strain. Due to their safety and probiotie effects,L, lactis have been studied emphasisly as a vector of recombinant protein expression. With the development of bio-teehnology, a series of expression systems have been constructed,where many heterogeneous proteins have been produced. The paper reviewed the recent progresses on Lactococcus lactis as cell factory for recombinant protein expression.

关 键 词：乳酸乳球菌 受体菌 外源蛋白 表达系统 

分 类 号：Q78[生物学—分子生物学]