蛋白芯片载体表面修饰对探针固定影响的研究  

Effect of Surface Modification for Protein Microarray on Immobilizing Protein Probe

作　　者：王涛[1] 郭子瑜[2] 张瑞[3] 聂利利[2] 贾芸芳[2] 邢克礼[1] 

机构地区：[1]天津医科大学生物医学工程学院,天津300070 [2]南开大学信息技术科学学院,天津300071 [3]天津医科大学代谢病医院,天津300070

出　　处：《生物技术通报》2010年第5期116-120,共5页Biotechnology Bulletin

基　　金：国家自然科学基金(60602002);天津市自然科学基金(08JCZDJC21700)

摘　　要：采用3-氨基丙基-三甲氧基硅烷((3-aminopropyl)trimethoxysilane,APTES)、戊二醛(glutaraldehyde,GA)、多聚-L-赖氨酸(poly-L-lysine,PLL)修饰芯片载体表面,对3种不同修饰方法制备的蛋白质芯片进行对比研究。将Cy3标记羊抗鼠IgG固定在修饰后片基上,选择蛋白探针的固定率作为检测指标;将小鼠IgG作为探针固定在芯片上,靶蛋白为Cy3标记羊抗鼠IgG,通过生物芯片扫描仪检测反应后荧光强度,选择蛋白探针的反应性作为检测指标,探讨制备蛋白质芯片较佳的表面修饰方法。结果显示,戊二醛修饰玻片对蛋白固定较好,有较高的反应活性,检测限较宽,但背景噪声较高。Glass slides were modified with （3-aminopropyl） trimethoxysilane, glutaraldehyde, poly-L-lysine to prepare protein mi- eroarrays. The optimal surface modification methods were evaluate and chosen for the preparation of protein mieroarray. The immobilizing proteins were labeled by Cy3, and attachment rate was evaluated. Then,the mouse IgG were printed on the modified glass slides, the target proteins were labeled by Cy3 ,and responsive activity of protein on microarrays were evaluated. The efficiency and the effects of immobilized proteins on the glass were compared. The result showed that the aldehyde modification yields high background,but have a larg detection limit, and the reactive activity of the immobilized proteins demonstrated superior.

关 键 词：蛋白质微阵列 表面修饰 固定率 反应性 

分 类 号：Q819[生物学—生物工程]