大鼠静脉移植物的组织因子表达  

Expression of tissue factor in venous bypass grafting of rats

作　　者：苏伟[1] 邱雪峰[1] 董念国[1] 史嘉玮[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管外科,湖北省武汉市430022

出　　处：《中国组织工程研究与临床康复》2009年第53期10439-10442,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖北省卫生厅科研基金(JX2B16)~~

摘　　要：背景:已有研究显示在动脉球囊损伤模型中,动脉中层平滑肌受损后组织因子表达迅速上调,缺血再灌注损伤也会导致内皮细胞组织因子表达增加。而对于静脉移植物中组织因子表达及其机制的研究较少。目的:观察静脉移植物管壁组织因子表达变化。设计、时间及地点:动物观察性实验,于2006-05/2008-05在华中科技大学同济医学院附属协和医院心血管外科实验室完成。材料:SD大鼠30只。方法:于颈内、颈外静脉汇合处结扎颈外静脉近心端,颈外静脉远心端在发出分支前结扎离断。以无损伤血管夹夹闭颈总动脉近心端和远心端,在两血管夹中间斜形切除5mm动脉。将动脉近心端和静脉远心端吻合两针并对抗牵引,再在两针之间的前后壁分别缝合三针;离断颈外静脉近心端,将其和颈总动脉远心端吻合。主要观察指标:免疫组织化学观察管壁组织因子、增殖细胞核抗原表达,同时检测标本蛋白抽提物组织因子活性,使用计算机图像分析系统分析内膜、中膜厚度,以对侧颈外静脉作为对照。结果:所有静脉移植物血管外膜均有组织因子表达,早期静脉移植物内膜、中膜组织因子表达明显增加,晚期静脉移植物内膜、中膜无组织因子表达;对照静脉内膜、中膜无组织因子表达。术后3d静脉移植物可观察到细胞增殖核抗原蛋白阳性表达,7d明显增高,14d达到最高。对照组静脉、早期和晚期静脉移植物血管蛋白抽提物组织因子活性无统计学意义。静脉移植物内膜在术后7,14,28d增生明显,中膜在术后14,28d增生明显。结论:不同时间点静脉移植物管壁组织因子表达变化与新内膜增生相关。BACKGROUND：Previous studies demonstrated that smooth muscle injury or ischemia/reperfusion injury result in tissue factor （TF） increasing.However,few reports concern the expression and mechanism of TF in venous bypass grafting.OBJECTIVE：To examine changes in TF protein expression in response to venous bypass grafting.DESIGN,TIME AND SETTING：The animal observation experiment was performed at the Department of Cardiovascular Surgery,Affiliated Union Hospital of Tongji Medical College,Huazhong University of Science and Technology from May 2006 to May 2008.MATERIALS：A total of 30 Sprague-Dawley （SD） rats.METHODS：Rats were underwent interposition bypass grafting of the common carotid artery via the ipsilateral external jugular vein.Namely,the proximal end of external jugular vein was ligated at the joints of external jugular vein and internal jugular vein,and the distal end of external jugular vein was ligated before branches.The proximal and distal ends of common carotid artery were occluded by artery clamp,and a 5 mm artery was removed.The proximal end of artery was anastomosed with distal end of artery,and the frontal wall was sutured with posterior wall.After that,the proximal end of external jugular vein was cut down and coincided with the distal end of common carotid artery.MAIN OUTCOME MEASURES：The expression of TF and proliferating cell nuclear antigen （PCNA） was detected by immunohistochemistry.Meantime,TF activity in vessel protein extracts was determined with TF activity assay kit,and the thickness of intima,media were calculated by computer imaging analysis system.The contralateral external jugular veins were served as the control.RESULTS：The adventitia of all vessels showed abundant TF staining.In early vein grafts,TF staining was markedly increased in the intima and media.However,intimal and media TF staining was absent in the contralateral control jugular veins and late vein grafts.The number of PCNA positive cells was increased in the vein grafts at day 3 after grafting,obviou

关 键 词：内膜增生 静脉移植物 血栓形成 

分 类 号：R318[医药卫生—生物医学工程]