南宁市2007～2008年诺如病毒感染所致成人腹泻散发病例的流行病学特征和诺如病毒基因型分布  

Norovirus Infection in Adults with Sporadic Gastroenteritis During 2007-2008 in Nanning Municipal

作　　者：谭冬梅[1] 刘巍[1] 邓丽丽[1] 唐振柱[1] 杨哲[1] 黄兆勇[1] 谢镇国[1] 方志锋[1] 杨进业[1] 董柏青[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《中国疫苗和免疫》2010年第2期132-135,共4页Chinese Journal of Vaccines and Immunization

基　　金：广西壮族自治区科学技术厅项目(桂科攻9732027;桂科攻0592007-4;桂科条08-05-01D);广西壮族自治区卫生厅科研课题(合同号:Z2008214)

摘　　要：目的研究诺如病毒(Norovirus,NV)感染所致成人腹泻散发病例的流行病学特征和NV基因型分布。方法对南宁市2007～2008年某医院门诊696例成人腹泻散发病例资料进行流行病学分析,并采集粪便标本,应用实时荧光定量逆转录-聚合酶链反应检测NV及其基因组别。随机选择部分阳性标本进行NV衣壳蛋白N/S区核苷酸序列测定,确定基因型。结果NV所致散发性成人腹泻在冬春季节呈现高发。在696份散发成人腹泻的粪便标本中,NV核酸检测阳性183份,总阳性率26.30%。其中180份(98.36%)NV属于基因Ⅱ组(GenogroupⅡ,GⅡ),另外3份(1.64%)为基因Ⅰ组(GⅠ)。随机选择10株GⅡNV进行核苷酸序列分析,它们均属于GⅡ.4基因型,而3株GⅠNV分属于GⅠ.2、GⅠ.4、GⅠ.8基因型。结论NV是南宁市2007～2008年成人腹泻散发病例的主要病原体,优势毒株为GⅡ.4型,也存在GⅠ型NV感染。Objective To investigate the epidemiological characteristic and genotype distribution of norovirus infection among adult patients with sporadic gastroenteritis in Nanning Municipal. Methods The epidemiological data of adult diarrhea patients in outpatient clinics of a hospital in Nanning City between January 2007 and December 2008 were analyzed.A total of 696 fecal specimens were collected from the patients and were screened for the presence of norovirus RNA by real-time reverse transcription-polymerase chain reaction.Some positive specimens were selected randomly and subjected for sequencing and phylogenetic analysis to identify genotypes.Results The sporadic norovirus infection in adults occurred preferentially during the cold season,and there was no statistical difference in the detection rate between age groups.183（26.30%）of 696 specimens were positive for norovirus RNA.Among them,180（98.36%）belonged to genogroupⅡ（GⅡ）and the other 3 （1.64%）belonged to genogroupⅠ（GⅠ）.The sequence analysis showed that all 10 GⅡstrains selected randomly belonged to genotype GⅡ4,while 3 GI strains were classified into three different genotypes： GⅠ2,GⅠ4 and GⅠ8.Conclusion The study showed that norovirus was an important etiologic agent of sporadic gastroenteritis among adults in Nanning City.Genotype GⅡ4 was the predominant genotype,and genotype GI existed sporadically.

关 键 词：诺如病毒 成人腹泻 散发病例 流行病学特征 基因型 

分 类 号：R373.25[医药卫生—病原生物学]