重寄生真菌盾壳霉产生几丁质酶的条件优化  

Optimization of Culture Conditions for Production of Chitinase by the Mycoparasite Coniothyrium minitans

作　　者：胡燕梅[1] 杨龙[2] 李国庆[2] 

机构地区：[1]武汉生物工程学院生物工程系,武汉430415 [2]华中农业大学湖北省作物病害监测与安全控制重点试验室,武汉430070

出　　处：《中国生物防治》2010年第2期167-173,共7页Chinese Journal of Biological Control

基　　金：国家自然科学基金(No.30070512)

摘　　要：本试验采用摇瓶培养的方法研究了盾壳霉产生胞外几丁质酶的条件。结果表明：培养液组分、通气状况、表面活性剂和草酸等因子对盾壳霉产生几丁质酶均有影响。改良的马铃薯蔗糖培养液（mPSB）较合成培养基SMCS更适宜作为几丁质酶产生的基础培养基,其几丁质酶产量达到616.8U/L;以mPSB为基础培养基,供试的9种碳源和7种氮源中5g/L葡萄糖和1g/L硝酸钾可使几丁质酶的产量分别达到756U/L和672U/L,较适宜几丁质酶的产生;生长曲线试验显示20℃培养15d几丁质酶产量达到高峰。另外,两种供试表面活性剂对几丁质酶的产生均有抑制作用;一定浓度的草酸溶液（0.1-3g/L）有助于盾壳霉几丁质酶的产生。Nutritional factors affecting production of chitinase by the mycoparasite Coniothyrium minitans were investigated in this study.The results showed that modified potato broth（mPSB） was more suitable than completely synthetic medium SMCS for C.minitans to produce chitinase and the production reached 616.8 U/L.Using mPSB as the basic medium,effect of caobon/nitrogen sources,aeration,surface active substances and oxalic acid on production of chitinase by C.minitans were characterized.The results showed that the optimum cultural conditions for chitinase production were glucose as carbon source at 5 g/L and potassium nitrate as nitrogen source at 1 g/L,which were suitable for chitinase production and led to the chitinase yield as 756 U/L and 672 U/L,respectively.Time course study revealed that the highest yield of chitinase occurred after shake-incubation for 15 d at 20℃.Two kinds of surface active substances were inhibitive for chitinase production,but 0.1-3 g/L oxalic acid could enhance chitinase production.

关 键 词：盾壳霉 几丁质酶 产酶条件 

分 类 号：S482.292[农业科学—农药学] TQ920.6[农业科学—植物保护]