白藜芦醇对大鼠急性脊髓损伤后MPO和NOS活性的影响  

Effects of Resveratrol on Activity of MPO and NOS after Acute Spinal Cord Injury of Rats

作　　者：赵训明[1] 刘世清[1] 梅红军 张弩[1] 马永刚[1] 邓明[1] 

机构地区：[1]武汉大学人民医院骨三科,武汉430060 [2]湖北省武汉市五医院骨科,武汉430060

出　　处：《科学技术与工程》2010年第14期3455-3458,共4页Science Technology and Engineering

摘　　要：为探讨白藜芦醇(Resveratrol,Res)对大鼠急性脊髓损伤后脊髓组织中髓过氧化物酶(MPO)和一氧化氮合酶(NOS)活性的影响,将120只SD大鼠随机区组分成4组:假手术组、损伤组、白藜芦醇(Res)组和甲基强的松龙(MPSS)组。假手术组切除椎板但不损伤脊髓,术后腹腔注射等量生理盐水,其余三组采用Allen's法以10g×5cm制作大鼠脊髓损伤模型,术后立即分别给予等量生理盐水Res(100mg/kg)和MPSS(100mg/kg)腹腔注射。术后8h、12h、24h、72h、168h取损伤脊髓标本,利用分光光度计测定MPO,NOS吸光度来检测其活性变化。结果SCI后脊髓组织中各时间点MPO和NOS活性均增加(P<0.01),损伤后3d达高峰;Res组和MPSS组在伤后各时间点MPO和NOS活性均小于损伤组(P<0.05);Res组和MPSS组比较,72h、168h时间点MPO活性无统计学意义;24h、72h、168h时间点NOS活性无统计学意义。说明白藜芦醇能抑制脊髓损伤后中性粒细胞浸润,抑制NOS的活性,减轻继发性脊髓损伤,对SCI具有保护和治疗作用。To study the effects of Resveratrol（Res）on activity of MPO and NOS after acute spinal cord injury of rats,120 SD rats were randomly divided into four groups.Rats in sham operated group had only daminectomy performed and nontraumatized spinal cord;rats in trauma group,Res group and MPSS group were injured by Allen＇s weight drop method（10 g×5 cm）.Then the normal saline,Resveratrol（Res,100 mg/kg）and Methylprednisolone（MPSS,100 mg/kg）were given intraperitoneally immediately respectively.The rats were divided into four subgroups for detecting the activity of MPO and NOS analysis at 8 h,12 h and 24 h,72 h,168 h after surgery.It is resulted that activity of MPO and NOS was increased obviously after SCI（P0.01）at each time point,and reach the peak at 72 h after injury;both Res group and MPSS group had less activity of MPO and NOS than trauma group（P0.05）.For MPO,there is no statistical difference between Res group and MPSS group at 72 h and 168 h.For NOS,there is no statistical difference between Res group and MPSS group at 24 h,72 h and 168 h.conclused that Res could protect spinal cord from injury in rats and relieve secondary spina1 cord injury.One of mechanisms is that Res can lessen the neutrophil and inhibit the activity of NOS.[

关 键 词：白藜芦醇 脊髓损伤 髓过氧化物酶 一氧化氮合酶 甲基强的松龙 

分 类 号：R651.2[医药卫生—外科学]