HPLC法检测蛇莓中熊果酸的含量  

DETERMINATION OF URSOLIC ACID IN THE HERBA DUCHESNEA INDICAE BY HPLC METHODS

作　　者：张建平 张羽飞[2] 包海花[2] 袁晓环[2] 

机构地区：[1]黑龙江省质量监督检测研究所,黑龙江哈尔滨150050 [2]牡丹江医学院抗纤维化生物治疗重点实验室,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2010年第2期25-27,共3页Journal of Mudanjiang Medical University

基　　金：黑龙江省卫生厅项目(2009-006)

摘　　要：目的:探讨HPLC测定蛇莓中熊果酸含量的方法。方法:采用Thermo ODS-2HYPERTIL C18(250×4.6mm,5μm)色谱柱;柱温为室温(26℃);流动相:NaH2PO47.8g/L-甲醇(14∶86);检测波长:210nm;流速:0.8mL/min。结果:熊果酸在1.780～5.340μg范围内呈良好线性关系(r=0.9965);平均回收率=98.7,RSD=0.92%(n=9)。结论:本测定方法简单、准确、可靠,可以作为评价蛇莓质量的有效方法。Objective：Investigate the method of the determination of Ursolic acid in the Herba Duchesnea Indicae.The chromatographic separation was performed on a Thermo ODS-2HYPER-TIL C18（250×4.6 mm,5μm）;Methods：The column warm was 25℃;the mobile phase consisting of NaH2PO47.8g/L-Methyl alcohol（14：86）;Examination wave length 210nm;Speed of flow：0.8mL/min.Results：The liner relationship of Ursolic acid was good within the range of 1.780～5.340μg（r=0.9965）.The average recovery was 98.7%（n=9） and RSD was 0.92%.Conclusion：The method is simple,accurate and reliable,and it can be used as an effective way to evaluate the quality of Duchesnea.

关 键 词：蛇莓 熊果酸 高效液相色谱 

分 类 号：R91[医药卫生—药学]