下丘脑中Ghrelin、Obestatin水平及其受体表达与糖尿病大鼠胃排空的关系  

Association of gastric emptying with ghrelin, obestatin and receptor （GHSR, GPR-39 ） in hypothalarnus of diabetic rats

作　　者：王金燕[1] 王利华[1] 魏良洲[1] 武军[1] 魏宁[1] 孔心涓[1] 田字彬[1] 

机构地区：[1]青岛大学医学院附属医院消化科,266003

出　　处：《中华医学杂志》2010年第16期1137-1140,共4页National Medical Journal of China

摘　　要：目的探讨糖尿病大鼠下丘脑胃促生长素（Ghrelin）、肥胖抑素（Obestatin）水平及受体表达与胃排空的关系。方法60只雄性Wistar大鼠随机等分为正常对照组、糖尿病组、胰岛素组。链脲佐菌素（STZ）造模，给药2周、6周后测胃排空及下丘脑Ghrelin、Obestatin、生长激素促分泌激素受体（GHSR）和孤儿蛋白受体39（GPR-39）。结果2周后，糖尿病和胰岛素组的胃排空率（％）（74±8、40±5）、Ghrelin（ng／g）（52±9、51±7）和Ghrelin／Obestatin（3．8±1．0、2．8±1．0）分别较正常组[32％±7％、（39±11）ng／g、2．1±0．8]高（均P〈0．05）。糖尿病组Obestatin（ng／g）（14．2±2．0）较正常组（21．7±4．7）低（P〈0．05），而GHSR（1．26±0．46）较正常组（0．77±0．21）高（P〈0．05）。胃排空率与Ghrelin、Ghrelin／Obestatin和GHSR／β-肌动蛋白（β-actin）正相关（r=0．49；r=0．63；r=0．73；P〈0．01），与Obestatin负相关（r=-0．74，P〈0．01）。6周后，糖尿病和胰岛素组胃排空率（78．97％±8．13％、44．06％±5．06％）均较正常组（35．06％±3．91％）高（均P〈0．01）。胃排空率与Ghrelin／Obestatin呈正相关（r=0．40，P〈0．05），未检测到GPR-39。结论高血糖早期胃排空加速可能受Ghrelin和GHSR表达增加影响，而持续期受Ghrelin／Obestatin增大影响。Objective To investigate the association of gastric emptying with ghrelin, obestatin and GHSR, GPR-39 in hypothalamus of diabetic rats. Methods Sixty Wistar rats were randomly divided into three groups ： a normal control group （ NC, n = 20）, a diabetes mellitus group （ DM, n = 20） induced by intraperitoneal injection of streptozotocin （STZ） and an insulin treated group （INS, n = 20）. After two and six weeks of STZ injection, gastric emptying was measured by intragastric administration of phenol red, ghrelin and obestatin in hypothalamus measured by ELISA （ enzyme-linked immunosorbent assay） and GHSR and GPR-39 by RT-PCR （reverse transcription-polymerase chain reaction） . Results After two weeks of STZ injection, gastric emptying （%） （74 ±8, 40 ±5）, ghrelin level（ng/g） （52± 9, 51 ±7）and ratio of ghrelin/obestatin（3.8 ± 1.0, 2. 8± 1.0）increased significantly in DM and INS groups compared to those in N C group [ 32% ± 7 %, （39 ± 11 ） ng/g, 2. 1 ±0. 8, all P 〈 0. 05 ]. Obestatin level（ng/g） （ 14. 2± 2.0）of hypothesis decreased significantly in DM group as compared to those in NC group（21.7 ± 4. 7 ）while GHSR/ [3-aetin increased significantly （ 1.26 ± 0. 46 vs 0. 77 ± 0. 21, P 〈 0. 05 ）. Gastric emptying was positively correlated with ghrelin, ghrelin/obestatin and GHSR/β-actin of hypothalamus （ r = 0. 49 ; r = 0. 63 ; r = 0. 73; P〈0. 01）. But there was a negative correlation with obestatin of hypothalamus （r = -0. 74, P 〈 0. 01 ）. After six weeks of STZ injection, gastric emptying （78.97% ± 8. 13% vs 44. 06% ± 5.06% ） increased significantly in DM and INS groups as compared to those in NC group（35.06%± 3. 91%, P 〈 0. 01 ）. Gastric emptying was positively correlated with ghrelin/obestatin of hypothalamus （ r = 0.40, P 〈 0. 05）. There was no detection of GPR-39 in hypothalamus. Conclusion The rapid gastric emptying may be due to the rising levels of ghrelin and GHSR in hypothalamus during early hyperglycem

关 键 词：糖尿病 胃排空 胃促生长素 肥胖抑素 

分 类 号：R285.5[医药卫生—中药学]