Genetic diversity and differentiation of the Dybowski's frog(Rana dybowskii) in Northeast China  

中国东北林蛙(Rana dybowskii)遗传多样性与分化研究(英文)

作　　者：张明[1] 贾学渊[2] 马建章[1] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]哈尔滨医科大学医学遗传学研究室,哈尔滨150081

出　　处：《Journal of Forestry Research》2010年第2期239-245,I0008,共8页林业研究（英文版）

摘　　要：The genetic diversity and population structure of the Dybowski＇s frog（Rana dybowskii） were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser Khingan Mountains and Changbai Mountains,China.Results showed that allele number of the 11 microsatellite loci was in the range of 2-10 in all populations,with the mean of 5.6.The average expected heterozygosity（HE） was 0.572,indicating a moderate polymorphism.The results of genetic differentiation coefficient（FST） showed that population genetic differentiation was significant between Changbai and Lesser Khingan Mountains（p〈0.001）.This result was verified further by Nei＇s genetic distance（DA） based on UPGMA phy-logenetic trees and by AMOVA analysis.In conclusion,the populations distributed in Lesser Khingan Mountains and Changbai Mountain are proposed to be two distinct management units（MUs） for their protection and management.本文利用11个微卫星多态位点检测东北林蛙(Rana dybowskii)的遗传多样性和种群遗传结构。6个东北林蛙种群的75个个体分布于中国的小兴安岭和长白山地区。11个位点在所有种群中得到的等位基因数为2～10个不等,平均5.6个;平均期望杂合度(HE)为0.572,属于中度多态。基因分化系数(FST)分析表明小兴安岭和长白山地区的种群间遗传分化显著(P<0.001),基于Nei's DA遗传距离构建的UPGMA系统树和AMOVA的结果进一步验证了结论的准确性。因此, 建议将分布于小兴安岭和长白山的东北林蛙划分为两个单独的管理单元加以保护和管理。图2表7参46。

关 键 词：Dybowski＇s frog（Rana dybowskii） genetic diversity genetic differentiation management units（MUs） MICROSATELLITE 

分 类 号：S966.35[农业科学—水产养殖]