沙眼衣原体激活MAPK通路而非NF-κB通路诱导IL-8生成  

Induction of IL-8 by Chlamydia trachomatis through MAPK pathway rather than NF-κB pathway

作　　者：陈凡[1] 程文[2] 张赛丹[1] 钟光明[3] 余平[2] 

机构地区：[1]中南大学湘雅医院心内科,长沙410008 [2]中南大学基础医学院免疫学系,长沙410078 [3]美国德克萨斯大学圣安东尼奥健康科学中心免疫学与微生物学系

出　　处：《中南大学学报（医学版）》2010年第4期307-313,共7页Journal of Central South University ：Medical Science

基　　金：美国国立卫生研究院基金~~

摘　　要：目的:研究沙眼衣原体诱导上皮细胞产生IL-8的信号通路。方法:应用Western印迹、免疫荧光技术和酶联免疫吸附实验(ELISA)检测沙眼衣原体感染Hela229细胞后诱导的细胞内、外IL-8表达情况,并对细胞内IL-8定位;Western印迹和免疫荧光技术检测沙眼衣原体感染后MAPK和NF-κB信号通路活化情况;利用MAPK和NF-κB信号通路化学抑制剂抑制MAPK和NF-κB通路,观察其对沙眼衣原体诱导IL-8生成的影响。结果:沙眼衣原体感染可诱导IL-8产生,呈时间依赖性;沙眼衣原体感染可激活MAPK/ERK和MAPK/p38信号通路,但不能激活NF-κB信号通路;利用化学抑制剂抑制ERK和p38通路可以抑制沙眼衣原体诱导上皮细胞产生IL-8。结论:沙眼衣原体诱导上皮细胞产生IL-8依赖于MAPK信号通路,但不依赖NF-κB信号通路。Objective To determine the signaling pathway required for Chlamydial induction of IL-8 expression in epithelial cells.Methods The production and localization of IL-8 in Chlamydia-infected Hela 229 cells were monitored using Western blot,immunoflourescence,and ELISA.Activation of MAPK and NF-κB signaling pathways were detected by Western blot and immunoflourescence.The effect of different signaling pathways on Chlamydia-induced Il-8 was measured by experiments of chemical inhibitors.Results IL-8 was induced by Chlamydia and was time-dependant.Chlamydial infection activated MAPK/ERK and MAPK/p38 pathways but not NF-κB pathway.Chlamydial induction of IL-8 was blocked by small molecule inhibitors targeting the ERK and p38 pathways.Conclusion Chlamydia-induced IL-8 in cervical epithelial cells,the natural target cell type of Chlamydia trachomatis infection,is dependent on MAPK pathway but not NF-κB pathway,which provides important information for further understanding the molecular mechanism of Chlamydia-induced inflammatory pathologies.

关 键 词：沙眼衣原体 MAPK通路 NF-ΚB通路 IL-8 

分 类 号：R518[医药卫生—内科学]