甲醛对雌性大鼠卵巢组织Fas凋亡途径相关基因表达的影响  

Effect of formaldehyde on expressions of Fas apoptosis pathway-related genes of ovary tissues in female rats

作　　者：彭国庆[1,2] 钟才高[1] 张琼[2] 谢颖[1] 龚凤英[2] 

机构地区：[1]中南大学公共卫生学院卫生毒理学系,长沙410078 [2]中南大学湘雅医院妇产科,长沙410008

出　　处：《中南大学学报（医学版）》2010年第4期341-345,共5页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(07JJ5094);湖南省科技厅科技计划项目(2007FJ4158;2007SK3028)~~

摘　　要：目的:观察甲醛对雌性大鼠卵巢组织Fas,caspase-8和caspase-3表达的影响,探讨甲醛对雌性大鼠卵巢毒性的分子机制。方法:将40只雌性SD大鼠随机分为正常对照组和3个不同浓度甲醛处理组,腹腔注射甲醛,剂量分别为20.0,2.0和0.2mg/kg,每天1次,连续14d后处死所有大鼠后取卵巢组织,用RT-PCR检测Fas和caspase-8mRNA表达,Western印迹检测Fas蛋白表达,分光光度法检测caspase-8和caspase-3的活性。结果:甲醛染毒组动物卵巢组织Fas与caspase-8mRNA表达以及caspase-8和caspase-3活性明显高于对照组,且随剂量的增加而增加(P<0.05)。结论:Fas基因表达与caspase活性的增强可能是甲醛诱导雌性动物卵巢毒性的重要机制。Objective To explore the mechanism of formaldehyde inducing ovarian toxicity in female rats by observing the effect of formaldehyde on the expression of Fas and caspase-8 mRNA,and the activity of caspase-3 and caspase-8 of ovary tissues in female rats.Methods Forty female Sprague-Dawley（SD） rats were randomly divided into a control group and 3 formaldehyde groups at different concentrations.The rats in the formaldehyde groups were intraperitoneally injected different doses of formaldehyde （20.0,2.0 and 0.2 mg/kg） continuously for 14 days.After 14 days,all rats were sacrificed and their ovaries were collected for detecting the expression of Fas and caspase-8 mRNA with RT-PCR,the protein expression of Fas with Western blot,and the activities of caspase-8 and caspase-3 with spectrophotometric method.Results Compared with the control group,the expression of Fas mRNA and its protein and caspase-8 mRNA and the activity of caspase-8 and caspase-3 of ovary tissues in the rats treated with formaldehyde significantly increased with dose （P〈0.05）.Conclusion The increase of Fas gene expression and the activity of caspase-8 and caspase-3 may be the important mechanism of ovarian toxicity induced by formaldehyde in female rats.

关 键 词：甲醛 大鼠 卵巢毒性 FAS caspase 

分 类 号：R114[医药卫生—卫生毒理学]