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作 者:唐标[1] 曹苏[1] 康焕菊[1] 朱翔[1] 陈秋萍[1] 盛俞[1]
出 处:《江苏医药》2010年第8期941-942,F0003,共3页Jiangsu Medical Journal
摘 要:目的探讨大鼠心肌微血管内皮细胞离体培养方法。方法选取1~2周龄的SD大鼠,无菌条件下快速取出心脏,彻底去除大血管、心房、右心室和心内外膜。用植块法培养心肌微血管内皮细胞。倒置显微镜下形态学观察结合免疫细胞化学方法检测第Ⅷ因子相关抗原和CD34对培养细胞进行鉴定。结果细胞呈单层贴壁生长,融合后呈"铺路石样";第Ⅷ因子相关抗原和CD34免疫细胞化学鉴定阳性。结论成功培养出纯度较高的大鼠心肌微血管内皮细胞,方法简便,可用于心血管疾病的研究。Objective To establish a method of culturing rat myocardial microvascular endothelial cells(MMVECs)in vetro.Methods SD rats(1-2 weeks of age)were selected.The heart was rapidly excised and the great vessels,atria,right ventricle,epicardium and endocardium were removed.Explants cultivation of cardiac tissues was used to obtain the primary cells.The purified cells were identified as vascular endothelial cells by morphology and positive immunocytochemistry for factor Ⅷ-related antigen and CD34.Results The cells were adherent,proliferated in a monolayer.Cobblestone like cells were observed under microscope after coalesced.The cells showed positive staining for factor Ⅷ-related antigen and CD34.Conclution MMVECs with high purity are obtained.The method is simple and convenient,and can be used to study the cardiovascular disease.
分 类 号:R541[医药卫生—心血管疾病]
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