丙肝病毒感染者血清抗核抗体与IL-21的检测  

The detection of anti-nuclear antibody and IL-21 in patients infected with hepatitis C virus

作　　者：邹敏[1] 李珉珉[2] 黄尚宝[2] 李晓娟[1] 周元平[3] 刘叔文[1] 

机构地区：[1]南方医科大学药学院,广东广州510515 [2]暨南大学第一附属医院,广东广州510630 [3]南方医科大学南方医院感染内科,广东广州510515

出　　处：《细胞与分子免疫学杂志》2010年第5期471-472,476,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：教育部新世纪优秀人才基金(NCET-06-0753);国家自然科学基金部分资助(30771899)

摘　　要：目的:检测丙肝病毒(HCV)感染者血清抗核抗体(ANA)、白细胞介素-21(IL-21)水平,探讨两者对HCV感染者肝功能的影响。方法:应用酶联免疫吸附法(ELISA)分别检测96例HCV-IgG阳性血清以及30例健康者血清的ANA与IL-21水平;用日立7600生化仪检测所有样本丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和白蛋白(ALB)的含量。结果:HCV感染者肝功能明显异常,IL-21水平较对照组低。ANA阳性患者大部分肝功能较好。ALT≤40U/LALT>40U/L感染组均低于对照组IL-21水平。结论:ANA、IL-21的产生有助于HCV感染者控制肝炎的发展,降低肝细胞的受损程度。AIM：To detect the serum anti-nuclear antibody （ANA） and interleukin-21 （IL-21） levels in patients infected with hepatitis C virus （HCV）,and study their impact on the liver function of HCV patients.METHODS：Enzyme-linked immunosorbent（ELISA） assays were used to detect the serum ANA and IL-21 levels in 96 cases of HCV-IgG positive sera,as well as 30 cases of HCV-IgG negative sera.Hitachi 7600 biochemical analyzer was used to test the ALT,AST and ALB of all sera samples.RESULTS：HCV patients have abnormal liver function significantly and their average IL-21 level was much lower than the control group.While the liver function of most ANA positive HCV patients was well-balanced.The IL-21 level of HCV patients with ALT≤40 U/L or ALT40 U/L were markedly reduced.CONCLUSION：The production of ANA and IL-21 do help to control the progress of hepetitis and reduce the damage to liver cells in HCV patients.

关 键 词：丙型肝炎 抗核抗体 IL-21 肝损伤 

分 类 号：R446.6[医药卫生—诊断学]