传染性单核细胞增多症患儿血清TNF-α和IL-18水平的动态变化  

Clinical significance of the changes of serum interleukin-18 and tumor necrosis factor-α levels in the children with infectious mononucleosis

作　　者：刘朝宙[1] 王伟[2] 周远大[3] 何海霞[3] 

机构地区：[1]湖南省儿童医院感染科,湖南长沙410007 [2]第三军医大学西南医院急救部,重庆400038 [3]重庆医科大学第一附属医院临床药理基地,基础医学院生物工程研究室,重庆400016

出　　处：《细胞与分子免疫学杂志》2010年第5期480-481,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：重庆市自然科学基金资助项目(CSTC2006EB5030)

摘　　要：目的:探讨儿童传染性单核细胞增多症(IM)不同时期血清细胞因子TNF-α、IL-18水平的变化。方法:选择2007-06/2009-06湖南省儿童医院收治的IM患儿72例为观察组,并随机选择同期健康儿童30例作为对照组。在病程急性期、病程1、3、6个月分别采集观察组患儿外周静脉血,健康对照组以同样方法采集静脉血,采用RIA检测TNF-α和ELISA检测IL-18水平。结果:观察组患儿IM急性期血清中TNF-α和IL-18含量均明显高于对照组(P<0.05),随病程时间推移,TNF-α和IL-18含量呈逐渐下降趋势,但各个病程时间点TNF-α和IL-18含量仍明显高于对照组(P<0.05)。观察组中45例轻症IM患儿与27例重症IM患儿相比较,重症IM患儿TNF-α,IL-18水平高于轻症IM患儿,两者比较有统计学差异(P<0.05)。结论:血清TNF-α和IL-18水平动态变化是判断IM患儿免疫状态和评估预后的重要指标。AIM：To study the dynamic changes of levels of serum interleukin-18（IL-18）and tumor necrosis factorα（TNF-α）in children with infectious mononucleosis（IM）.METHODS：72 patients with IM were analyzed the expression of TNF-α and IL-18 by RIA and ELISA respectively in acute phase,convalescent phase（the first month,the third month,the sixth month）and the control group.RESULTS：The levels of serum TNF-α and IL-18 in patients with IM were significantly higher than that in control（P0.05）.The Serum levels of TNF-α and IL-18 decreased with time and in patients at stage were significantly higher than that in control（P0.05）.The levels of serum cytokine were correlated with the state of illness.CONCLUSION：The serum cytokine TNF-α and IL-18 may be regarded as index to reflect the state of immunity and prognosis of patients with IM.

关 键 词：传染性单核细胞增多症 肿瘤坏死因子-Α 白介素-18 

分 类 号：R392.11[医药卫生—免疫学]