低聚葡萄籽原花青素对异丙肾上腺素诱导大鼠心室重构的影响  

Effects of oligomeric grape seed proanthocyanidins on isoproterenol-induced cardiac remodeling in rats

作　　者：左友梅[1] 高杉[2] 曹建飞[2] 刘晓雨[2] 於洪建[3] 张野[1] 

机构地区：[1]安徽医科大学第二附属医院麻醉科,安徽合肥230601 [2]安徽医科大学基础医学院药理学教研室抗炎免疫药理学省部共建教育部重点实验室,安徽合肥230032 [3]天津市尖峰天然产物研究开发有限公司,天津300457

出　　处：《药学学报》2010年第5期565-570,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30672032);安徽省优秀青年基金资助项目(8040106814);安徽医科大学博士科研经费资助项目(XJ200709)

摘　　要：研究低聚葡萄籽原花青素(oligomeric grape seed proanthoc yanidins,GSP)对异丙肾上腺素(isoproterenol,ISO)致大鼠心室重构的保护作用并初步探讨其机制。采用皮下注射ISO致大鼠心室重构模型,以GSP(50,100及150mg·kg-1)灌胃给药,PowerLab监测大鼠心功能,计算大鼠全心重量指数(HW/BW)和左心重量指数(LVW/BW),观察心肌病理学改变,测定左心室心肌组织中羟脯氨酸(Hyp)含量、血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果表明,与空白对照组相比,ISO组大鼠心功能明显受损,心脏重量指数HW/BW和LVW/BW、心肌细胞横截面积(CSA)、心肌间质胶原容积分数(CVF)和心肌血管周围胶原面积(PVCA)、心肌组织Hyp含量明显升高,血清SOD活性下降,MDA含量升高。与ISO组相比,GSP能明显改善心功能,降低心脏HW/BW和LVW/BW、CSA、CVF、PVCA和左心室心肌组织中Hyp含量,增加血清SOD活性,降低血清MDA含量。GSP对ISO诱导的大鼠心室重构具有明显的逆转作用,其机制可能与抗氧化应激,提高机体的抗氧化能力有关。The purpose of this study is to evaluate the effect of oligomeric grape seed proanthocyanidins （GSP） on isoproterenol （ISO）-induced cardiac remodeling in rats.ISO was given subcutaneously （5 mg·kg^-1,sc,7 days） to induce cardiac remodeling in rats.Therapeutic groups were given GSP （50,100,and 150 mg·kg^-1） after ISO treatment.After 2 weeks intervention,heart rate （HR）,left ventricular systolic pressure （LVSP）,left ventricular end-diastolic pressure （LVEDP）,rate of rise of left ventricular pressure （±dp/dtmax） were examined.The myocardial hypertrophy index was expressed as heart weight/body weight （HW/BW） and left ventricle weight/body weight （LVW/BW）,the histological changes were investigated by HE and Van Gieson stain.SOD activity and MDA content in serum,contents of hydroxyproline （Hyp） in the left ventricular tissue were assayed by xanthinoxidase method,thiobarbituric acid （TBA） method and alkaline hydrolysis method,respectively.After the onset of ISO-treatment,GSP therapy potently improved cardiac function,inhibited myocardial hypertrophy,improved cardiac pathology change,decreased the myocardial cross-section area （CSA）,collagen volume fraction （CVF） and perivascular circumferential collagen area （PVCA）,reduced the content of Hyp in the left ventricular tissue,inhibited the decrease of SOD activity and increase of MDA content in serum.GSP possess protective effect against ISO induced cardiac remodeling in rats,this may be related to reducing the oxidative stress and improving antioxidant capacity.

关 键 词：低聚葡萄籽原花青素 异丙肾上腺素 心室重构 氧化应激 

分 类 号：R285[医药卫生—中药学]