内标法测定烟碱微透析回收率的可行性研究  

Internal standard method to determine the recovery of nicotine in microdialysis

作　　者：凌家俊[1] 陈翔[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《药学学报》2010年第5期632-635,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30772791)

摘　　要：研究利用内标法测定烟碱微透析回收率的可行性。通过测定磷酸可待因与烟碱在不同情况下的体外回收率(或释放率),考察它们之间的关系。考察探针周围烟碱浓度对两种物质的体外回收率(或释放率)及它们之间比值的影响。分别用内标法及反透析法测定烟碱大鼠体外血浆蛋白结合率。结果显示,磷酸可待因的释放率与烟碱的回收率之间的比例较稳定,磷酸可待因的释放率不因探针周围烟碱的浓度而改变。内标法及反透析法测得的血浆蛋白结合率相差不大。内标法可作为体内微透析研究中烟碱回收率的测定方法,磷酸可待因可作为该方法中的内标物。The paper reports the evaluation of the feasibility of using internal standard method for the determination of nicotine recovery in microdialysis in vitro.This in vitro experiment included two conditions.Nicotine and codeine phosphate were dissolved in Ringer’s solution.Nicotine,codeine phosphate and the mixture of them were perfused through the CMA30 linear probe separately to calculate the proportion of the recovery （or delivery） of nicotine to that of codeine phosphate.And then codeine was perfused through the probe which was immersed in nicotine solution with different concentrations to calculate the proportion,too.In another condition nicotine was dissolved in rat plasma.The rat plasma protein binding rate was determined by using retrodialysis and internal standard method in vitro.The results are as follows： the proportion of the recovery （or delivery） of nicotine to that of codeine phosphate was fairly stable.The delivery of codeine was independent of nicotine concentration in the external medium.Protein binding rate determined by retrodialysis was almost the same as that determined by internal standard method.It suggests that the internal standard method is an effective way in the determination of nicotine recovery and codeine phosphate can be used as the internal standard.

关 键 词：烟碱 微透析 内标法 

分 类 号：R917[医药卫生—药物分析学]