丙硫氧嘧啶对大鼠甲状腺TPO、Tg mRNA表达的影响  

Effect of PTU on the Expression of Thyroid TPO,Tg mRNA in Rats

作　　者：商维维[1] 罗建[1] 程薇波[1] 黄芹[2] 王文冬[1] 

机构地区：[1]四川大学华西公共卫生学院病理学教研室,成都610041 [2]四川大学华西第二医院病理科,成都610041

出　　处：《四川大学学报（医学版）》2010年第3期428-431,共4页Journal of Sichuan University(Medical Sciences)

基　　金：成都市科技局科技计划项目(05GGSF091)资助

摘　　要：目的探讨甲状腺干扰物丙硫氧嘧啶(propylthiouracil,PTU)对大鼠甲状腺功能影响的作用机理及代偿进程。方法雄性SD成年大鼠140只,PTU〔5.0 mg/(kg.d)〕灌胃染毒,分别设染毒3 d、6 d、9 d、12 d、15 d、18 d、21 d染毒组和同期对照组,每组10只大鼠,在染毒期满后处死动物,采用RT-PCR检测甲状腺过氧化物酶(TPO)和甲状腺球蛋白(Tg)mRNA的表达,电化学发光法检测血清甲状腺激素水平。结果与对照组相比,各染毒组TPOmRNA的表达增强,TgmRNA的表达减弱(P均<0.05)。各染毒组血清TT3、TT4呈下降趋势,TSH呈上升趋势,且与对照组相比,从染毒6 d开始,血清TT3、TT4、TSH差异有统计学意义(P<0.05)。结论 PTU对甲状腺的干扰机制,可能涉及抑制Tg的转录。TSH水平增高对TPO基因的转录刺激作用,不能起到充分的代偿作用。Objective To explore the mechanism and the compensation proceeding of thyroid hyperplasia caused by PTU.Methods PTU was administered to rats by gavage at a dose of 5.0 mg/kg B.W for 3,6,9,12,15,18,21 days respectively.All animals were sacrificed after the last dosage,and the expression of TPO and Tg in thyroid was detected by RTPCR.The serum thyroid hormones were measured by chemoluminescence.Results Compared with the control group,the TPO mRNA levels of PTU groups were increased,while the Tg mRNA levels were decreased significantly.Serum TT3,TT4 in rats treated with PTU demonstrated a descending trend,while serum TSH showed an ascending trend,and the significant differences were observed after 6 days treating with PTU.Conclusion The interference of PTU on thyroid may relate to inhibiton of Tg gene transcription.The enhancement of TPO gene transcription can＇t compensate the thyroid function sufficiently.

关 键 词：丙硫氧嘧啶 甲状腺过氧化物酶 甲状腺球蛋白 

分 类 号：R965[医药卫生—药理学]