外源性重组人促红细胞生成素在宫内缺血缺氧环境中对胎盘屏障通透性的实验研究  

Permeability of Exogenetic Recombine Human Erythropoietin Through Placenta Barrier to Fetal Rats with Transient Uteroplacental Ischemia

作　　者：周俊[1] 马玉姗[1] 柳惠[1] 林雪梅[1] 

机构地区：[1]四川大学华西第二医院麻醉科,成都610041

出　　处：《四川大学学报（医学版）》2010年第3期445-447,共3页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅项目(编号2008JY0028-1)资助

摘　　要：目的探讨重组人促红细胞生成素(recombine human erythropoietin,rhEPO)在宫内缺血缺氧时能否通过胎盘屏障及血脑屏障。方法孕19 d(孕晚期)SD大鼠,分为3组:缺血缺氧组(I/R)、假手术组(Sham)和rhEPO治疗组(Treat)。钳夹双侧子宫血管制备I/R模型,经尾静脉分别注射生理盐水及125I标记的rhEPO(5000U/kg),术后30 min、3 h、6 h和24 h后取胎盘、羊水及胎鼠脑、心、肝、肺和肾,检测和比较各组织125I-rhEPO的放射比活性。结果 I/R组仅能测得少量的放射活性〔(1.26±0.28)pg/g〕。假手术组和rhEPO治疗组胎盘、羊水及胎鼠各组织的125I-rhEPO含量在再灌注30 min、3 h和6 h呈逐渐升高趋势,再灌注6 h与再灌注30 min比较差异有统计学意义(P<0.01)。组织之间进行相互比较,胎盘125I-rhEPO含量最多,胎盘与其它组织相比,再灌注30min、3 h、6 h和24 h各时点差异均有统计学意义(P<0.05)。rhEPO治疗组与假手术组各时点相比,rhEPO治疗组胎鼠脑组织内的125I-rhEPO含量从再灌注3 h开始更多,在再灌注6 h时达到峰值(P<0.05)。结论在宫内缺血缺氧时,胎盘屏障及胎鼠的血脑屏障对外源性rhEPO的通透性增加。Objective To investigate whether recombine human erythropoietin can cross the placenta barrier in rats with transient uteroplacental ischemia.Methods Rats on day 19 of pregnancy were divided into ischemia-reperfusion group,sham-operated group and rhEPO treated group. Fetal ischemia was induced by bilateral occlusion of the utero-ovarian artery for 20 minutes.^125I-rhEPO or saline were administered intravenously 30 min before the induction of hypoxic-ischemic injury.Fetal rat organs were removed to measure the radioactivity post injury.Results A small amount of radioactive activity（1.26±0.28） pg/g was detected in the fetal rats with ischemia-reperfusion.^125I-rhEPO radioactivity increased gradually with time in the placenta,amniotic fluid and fetal tissues of vital organs in the rats of sham-operated group and rhEPO treatment group.There were significant differences in ^125I-rhEPO between placental organs and other organs （P〈0.05）.The permeability of ^125I-rhEPO through the blood brain barrier changed with reperfusion time and peaked about 6 h after reperfusion,and significant differences were found between rhEPO treatment group and sham operation group（P〈0.05）.Conclusion Exogenous rhEPO can cross the placenta barrier and blood-brain barrier and reach hypoxic-ischemic fetal rats.

关 键 词：重组人促红细胞生成素 缺血缺氧 胎盘屏障 血脑屏障 通透性 

分 类 号：R714.2[医药卫生—妇产科学]