阿霉素肾病大鼠肾小球中足细胞数量及nephrin、VEGF的表达变化  

Podocyte Injury and Expression of Nephrin and VEGF in Rat Nephrosis Model Induced by Adriamycin

作　　者：杨维娜[1] 任淑婷[2] 钱亦华[1] 成少利[3] 靳辉[1] 刘勇[1] 于琳华[2] 

机构地区：[1]西安交通大学医学院人体解剖与组织胚胎学系,西安710061 [2]西安交通大学医学院病理学系,西安710061 [3]西安交通大学医学院形态中心,西安710061

出　　处：《四川大学学报（医学版）》2010年第3期458-463,共6页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨足细胞的数量及nephrin、VEGF在阿霉素(ADR)肾病大鼠肾小球中的表达变化及意义。方法建立ADR诱导的大鼠肾病模型,于不同实验时间点检测血尿生化指标;光镜、电镜观察肾脏病理学改变;免疫组化技术观察足细胞数量、nephrin、VEGF的表达变化。结果肾病模型组大鼠在第2次注射ADR后1周末足细胞nephrin表达减弱(P<0.05);8周末肾小球VEGF表达增强(P<0.05)、足细胞数量减少(P<0.05);nephrin、足细胞数量与24 h尿蛋白定量、尿素氮、血肌酐呈负相关;VEGF与24 h尿蛋白定量、尿素氮、血肌酐呈正相关。结论足细胞nephrin的表达减少和分布方式改变可能是大量蛋白尿发生的重要因素,VEGF既参与蛋白尿的产生,又参与肾小球硬化。Objective To investigate podocyte injury and the expression of nephrin and VEGF in rat nephrosis model induced by adriamycin.Methods The rat adriamycin induced nephrosis model was established,while the biochemical indicators in blood and urine were measured and the pathological changes of the renal tissue were evaluated by light microscope and electron microscope.The podocyte number was counted,and the expression levels of nephrin,VEGF were examined at different time by means of immunohistochemistry.Results After second injected with adriamycin,the model group nephrin presented a weak signal in the end of the first week（P〈0.05）,and the expression of VEGF started to increase at the end of the eighth week（P〈0.05）.The podocyte number decreased at the end of the eighth week（P〈0.05）.The expression of nephrin and the number of podocyte were negatively correlated with the 24-hour urine protein,blood urea nitrogen and serum creatinine;while the expression of VEGF was positively correlated with the 24-hour urine protein,blood urea nitrogen and serum creatinine.Conclusion The decrease of nephrin expression and the change of its distribution might be the significant factors resulting in considerable proteinuria.VEGF participated in the process of proteinuria and glomerular sclerosis in the development of rat adriamycin nephrosis.

关 键 词：阿霉素肾病 NEPHRIN VEGF 足细胞 

分 类 号：R692[医药卫生—泌尿科学]