人防御素α5、人防御素β2在不孕患者输卵管的表达及意义  

The Relationship Between HαD-5 and HβD-2 in Infertile Women's Fimbriae Tubes with Adhesions and Atresias

作　　者：胡诗婉[1] 毛毳[1] 张丹[1] 兰珍[1] 王成芳[1] 罗书[1] 

机构地区：[1]四川大学华西第二医院生殖内分泌科,成都610041

出　　处：《四川大学学报（医学版）》2010年第3期480-482,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的通过分析粘连、闭锁的输卵管伞和正常输卵管伞中人防御素α5(humanαdefensin-5,HαD-5)、人防御素β2(humanβdefensin-2,HβD-2)的相关性,初步探讨HαD-5和HβD-2在输卵管伞粘连发生中的作用与意义,为临床防治输卵管伞粘连闭锁引起的不孕提供新的思路。方法收集临床手术切除的输卵管伞组织,将输卵管伞分为粘连组(30例)和非粘连组(30例),应用免疫组化SP法检测HαD-5和HβD-2的表达情况,分析二者的相关性。结果免疫组化染色结果显示HαD-5、HβD-2两种因子在粘连组的表达强于不粘连组,且差异有统计学意义(P<0.05)。不粘连组中HαD-5和HβD-2的表达呈正相关(r=0.404,P<0.05)。粘连组中HαD-5和HβD-2的表达无相关性(P=0.089)。结论 HαD-5、HβD-2在炎症发生时表达明显增强,在正常情况下,两者存在相互促进的协同作用,这种协同作用的破坏可能是粘连形成的原因之一。Objective To investigate the expression and relationship of human α defensin-5（HαD-5） and human β defensin-2（HβD-2） in human fimbriae tubes with adhensions and atresias.Methods The tissue samples were collected from 30 human fimbriae tubes with adhensions and atresias,and 30 cases without adhensions and atresias.The expression of HαD-5 and HβD-2 in fimbriae tube tissue were measured by immunohistochemical SP methods.Image pro-plus 6.0 software was used to test the average IOD value of positive staining.Differences of HαD-5 and HβD-2 expressions were analyzed by independent-samples T test.The relationship between HαD-5 and HβD-2 was analyzed by Pearson correlation.Results We found that HαD-5 and HβD-2 mainly expressed in the epithelial cells which face to lumen,mostly in the cytochylema.Both two groups expressed HαD-5 and HβD2,but the degrees were different.Compared with human fimbriae tubes without adhesions or atresias,the expressions of HαD-5 and HβD-2 increased significantly in adhesion cases（PHαD-5=0.000,PHβD-2=0.02）.In the group without adhesion,there was a positive correlation between HαD-5 and HβD-2（r=0.404,P=0.027）,while in the adhesion group,there was no correlation between HαD-5 and HβD-2（P=0.089）.Conclusion HαD-5 and HβD-2 may protect fimbriae tubes during the pathological process of microorganisms attack.

关 键 词：人防御素α5 人防御素β2 免疫组织化学 相关性 

分 类 号：R711.6[医药卫生—妇产科学]