通心络胶囊干预载脂蛋白E基因敲除小鼠CEC、ET机理的研究  

Mechanism Research of Tongxinluo Capsule on CEC and ET of the Apolipoprotein E Gene Knockout Mice

作　　者：李七一[1] 韩旭[1] 夏卫军[1] 赖仁胜[1] 陈晓虎[1] 郭宏敏[1] 刘福明[1] 赵惠[1] 朱波[1] 

机构地区：[1]江苏省中医院,南京210029

出　　处：《中国中医急症》2010年第5期807-809,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：江苏省自然科学基金课题(NoBK2006157)

摘　　要：目的通过观察通心络胶囊对载脂蛋白E(ApoE)基因敲除小鼠循环内皮细胞(CEC)、小鼠内皮素(ET)水平的干预变化,探究其对ApoE基因敲除小鼠冠状动脉粥样硬化(AS)的调节作用和机理。方法将40只ApoE基因敲除小鼠作为实验组,建立冠状AS模型,随机分为模型组等5组。结果通心络胶囊干预后,通心络胶囊高、中、低剂量组和辛伐他汀组、模型组相比,通心络胶囊高剂量组在造模后第4、8、12周血中CEC均明显低于模型组,中剂量组在造模后第4周和第12周时血中CEC与模型组相比显著降低。模型组小鼠血清ET含量高于正常组,通心络胶囊各剂量组小鼠血清ET含量均低于模型组。结论通心络胶囊能降低ApoE基因敲除小鼠CEC、ET水平,具有调节血管内皮细胞功能和抑制炎症因子的作用。Objective：To observe the Tongxinluo Capsule on CEC and ET of the apolipoprotein E gene knockout mice and to detect the regulation of apolipoprotein E gene in the coronary arteriosclerosis mice.Methods：40 Apolipoprotein E gene knockout mice were established the models of coronary atherosclerosis.They were randomly divided into 5 groups.Results：In the 4th week,the 8th week and the 12th week after the models established,the amount of CEC in the high dose group was less significantly than that in the model group.In the 4th week and 12th week after models established,the level of CEC in the middle dose group was also lower obviously than that in the model group.The content of ET in the model group was higher significantly than that in the normal group.Also,these levels in three dose of Tongxinluo Capsule groups were less remarkably than those in the model group.Conclusion：Tongxinluo Capsule can decrease CEC and ET of apolipoprotein E gene knockout mice.It can not only regulate vascular endothelial cells,but also inhibit inflammation factors.

关 键 词：通心络胶囊 APOE基因敲除小鼠 循环内细胞 内皮素 

分 类 号：R285.5[医药卫生—中药学]