垃圾渗滤液处理系统中微生物群落结构变化研究  

Variation of Microbial Community Structures in Landfill Leachate Treatment System

作　　者：邵军[1,2] 孙海美[1,2] 孙卫玲[2] 李振山[2] 

机构地区：[1]北京大学深圳研究生院环境与能源学院、城市人居环境科学与技术重点实验室,深圳518055 [2]北京大学环境科学与工程学院、水沙科学教育部重点实验室,北京100871

出　　处：《北京大学学报（自然科学版）》2010年第3期435-441,共7页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：采用基于PCR的变性梯度凝胶电泳(PCR-DGGE)和real-time PCR方法,分析了深圳市某垃圾渗滤液处理系统中总细菌和硝化细菌的生物量及群落结构变化,探究生物量与水质变化间的关系及环境因素对微生物群落结构的影响。结果表明,该垃圾渗滤液处理系统具有很好的氨氮和总有机碳(TOC)去除效果,总去除率分别为99.9%和91.4%。在垃圾渗滤液处理系统中,总细菌、氨氧化菌(AOB)、亚硝酸盐氧化菌(NOB)的生物量与TOC及氨氮浓度没有显著相关性(p>0.05);溶解氧不影响AOB生物量,但明显影响NOB生物量。微生物群落结构及多维尺度(DMS)分析表明:在该废水处理系统中,DO浓度对总细菌和NOB微生物群落结构有重要影响;而氨氮浓度是影响AOB群落结构的关键因素。To disclose the mechanism of N-removal in a full-scale landfill leachate treatment system （Shenzhen）, community structure of total bacteria and nitrifier in this system were explored by PCR-DGGE and real-time PCR. The influence of environmental factors on microbial community structure was analyzed. The results show that 91.4% of TOC and 99.9% of NH4^＋ -N were removed during the whole process. The quantitative assay show that the numbers of total bacteria, AOB,and NOB had no significant relationship with the concentrations of TOC and ammonia （p 〉 0. 05）. Dissolved oxygen （DO） had no effect on the number of AOB ,but had great influence on the number of NOB. Microbial community structure and DMS analysis show that the DO concentration affected the community structure of total bacteria and NOB, while ammonia concentration determined the community structure of AOB.

关 键 词：群落结构 生物多样性 硝化细菌 变性梯度凝胶电泳 定量PCR 

分 类 号：X703[环境科学与工程—环境工程]