脂氧素A4对重度子癎前期患者外周血单核细胞体外分泌白细胞介素-1β的影响及其与胞浆内游离钙浓度的关系  

Effect of lipoxin A4 on interleukin-1β production of human monocytes from severe preeclamptic women and its relationship with cytoplasm free calcium concentration--an in vitro study

作　　者：王建芳[1] 黄引平[1] 黄艳君[1] 刘小利[1] 周洁[1] 王静[1] 

机构地区：[1]浙江省温州医学院附属第一医院妇产科,325000

出　　处：《中华围产医学杂志》2010年第3期191-195,共5页Chinese Journal of Perinatal Medicine

基　　金：国家高等学校博士点学科点专项科研基金（200803430002）

摘　　要：目的探讨脂氧素A4（1ipoxin A4，LXA4）对重度子癎前期（severe preeclampsia，SPE）患者外周血单核细胞体外分泌白细胞介素－1β（interleukin，IL-1β）的影响及与胞浆游离钙浓度（[Ca^2＋]i）的关系。方法收集温州医学院附属第一医院产科2008年10月至2009年5月收治的15例SPE患者（SPE组）和20例正常孕妇（正常妊娠组）的外周静脉血，分离外周血单核细胞进行体外培养。将不同浓度LXA4（0、10、100nmol／L）加入SPE组单核细胞体外培养后，采用酶联免疫吸附实验测定培养上清液中1I，1口的浓度。钙离子荧光探针Fluo-3／AM负载单核细胞后，用激光共聚焦扫描显微镜动态检测单核细胞内[Ca^2＋]i的变化。结果（1）体外在0、10、100nmol／L浓度LXA4的作用下，SPE组外周血单核细胞培养上清液中IL-1β水平分别为（63．16±8．20）pg／L、（53．71±8．08）pg／L、（3．16±6．89）pg／L。LXA4呈剂量依赖性抑制了SPE单核细胞分泌IL-1β（P〈0．05），而其对正常妊娠组IL-1β的分泌无影响[分别为（19．22±7．43）pg／L、（16．99±6．32）pg／L、（15．18±5．24）pg／L]（P〉0．05）。（2）SPE组外周血单核细胞[Ca^2＋]i为1028．09±160．52，较正常妊娠组（323．61±87．86）明显升高（P〈0．05）。而在100nmol／L LXA4作用下SPE组单核细胞[Ca^2＋]i为409．67±116．73，较0nmol／L LXA4作用下SPE组单核细胞[Ca^2＋]i明显下降（P％0．05）。结论LXA。体外能明显抑制SPE患者单核细胞分泌IL—1β，其机制可能与降低细胞内[Ca^2＋]i有关。Objective To investigate the effect of lipoxin A4 （LXA4） on interleukin-1β （IL-1β） production of monocytes in maternal peripheral blood from severe preeclampsia women and its relationship with cytosolic free calcium （[Ca^2＋]i） concentration. Methods Peripheral venous blood was drawn from 15 women with severe preeclampsia （SPE group） and 20 normal pregnant women （control group） who were admitted to the First Affiliated Hospital of Wenzhou Medical College from October 2008 to May 2009. Monocytes were obtained from peripheral blood with Ficoll density gradient centrifugation and then were suspended in RPMI 1640 culture supplemented with LXA4 at the final concentrations of 0,10 and 100 nmol/L, respectively. IL-1β levels in monocytes supernatant were detected by enzyme linked immunosorbent assay. The cytoplasma [Ca^2＋]i of cultured monocytes and its variations affected by LXA4 were measured by laser scanning confocal microscope. Results （1） After incubation with different concentrations of LXA4 （0,10,100 nmol/L） for 24 h, the levels of IL-1β in SPE group were （63.16±8.20） pg/L, （53.71±8.08） pg/L and （43.16±6.89）pg/L,respectively, indicating a significant inhibition effect on IL-11？ level in a dose-dependent manner （P〈0.05）. The IL-1β levels in the control group were （19.22±7.43） pg/L, （16.99±6.32） pg/L and （15.18±5.24） pg/L, correspondingly （P〉0.05）. （2） Without LXA4, the [Ca^2＋]i concentrations of monocytes in the SPE group were higher than that of the control （1028.09±160.52 vs 323.61± 87.86, P〈0.05）. After treatment with 100 nmol/L LXA4 ,the [Ca^2＋]i concentration of monocytes significantly decreased in the SPE group （409.67±116.73, P〈0.05）. Conclusion In vitro LXA4 may inhibit the IL-1β production in monocytes of SPE patients through decrease of the cytoplama [Ca^2＋]i concentration.

关 键 词：脂氧素类 先兆子癎 单核细胞 白细胞介素1G 钙 

分 类 号：R714.245[医药卫生—妇产科学]