小苦苣菜水提取物中木犀草苷的含量测定  

Determination of Galuteolin in Water-Extract of Ixerlidium Chinense(Thunb.) by HPLC

作　　者：梁惠珍[1] 王丽芳[2] 裴妙荣[1] 

机构地区：[1]山西中医学院 [2]山西中医学院附属第二中医院

出　　处：《山西中医》2010年第5期46-47,共2页Shanxi Journal of Traditional Chinese Medicine

摘　　要：目的：采用HPLC法测定小苦苣菜水提取物中木犀草苷的含量。方法：HPLC法,Diamonsil C18柱（4.6 mm×250 mm,5μm）;流动相：乙腈-0.5%冰乙酸水溶液（20∶80）;流速：1.0 mL.min-1;检测波长：350 nm。结果：木犀草苷含量在0.119 2~0.953 6μg范围内线性关系良好,相关系数r=0.9999,平均回收率为98.94%,RSD=1.13%（n=6）。结论：该方法简便,可靠,可用于小苦苣菜药材的质量控制。Objective： To determine galuteolin in water-extract of Ixerlidium chinense（Thunb.）by HPLC.Method： HPLC was used in the quantitative analysis of galuteolin in the sample.The separation was performed on Diamonsil C18（4.6 mm ×250 mm,5 μm） column with a mixture of acetonitrile-0.5 % acetic acid glacial（20∶80） as mobile phase.The detection wavelength was set at 350 nm and the flow rate was 1.0 mL·min-1.Result： The linear range of galuteolin was 0.119 2 μg-0.953 6 μg（r=0.999 9）,the average recovery was 98.94%,RSD = 1.13%（n=6）.Conclusion： The method is simple,reliable and good in determining galuteolin in Ixerlidium chinense（Thunb.）.

关 键 词：小苦苣菜 木犀草苷 HPLC 

分 类 号：R282.71[医药卫生—中药学]