缓苗期遮荫对雌雄银杏幼苗抗氧化酶系统及生化物质含量的影响  

Effects of Shades on Activities in Antioxidant Enzymes and Biochemistry Substances Content of Male and Female Ginkgo biloba L. Leaf During the Rejuvenation Period

作　　者：杨妤[1,2] 田茂洁[1,2] 蒋雪梅[1,2] 韦伟[1,2] 黄尤优[1,2] 曾永海 赵蓉 

机构地区：[1]西华师范大学生命科学学院 [2]西南野生动植物资源保护教育部重点实验室,四川省南充市637009 [3]冕宁县冶勒自然保护区,四川省冕宁县615600

出　　处：《光谱实验室》2010年第3期918-922,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：四川省重点学科建设项目(SZD0420);大学生科技创新基金(42708053)

摘　　要：本实验采用双因素完全随机设计,对银杏(Gingko biloba L.)移栽苗处于缓苗期的衰老叶片进行57%遮荫处理。利用紫外分光光度法测得结果:(1)遮荫处理降低了银杏植株的SOD活性,雌株降低了32.69%,雄株降低了27.51%;(2)遮荫处理降低了POD活性:雌株降低了76.14%,雄株降低了53.88%;(3)雌雄株的可溶性蛋白含量也分别降低12.02%和2.74%;(4)遮荫后雌雄株MDA的含量分别提高了79.06%和51.34%。雌株对遮荫的反应更为敏感,各项指标变化均大于雄株,因此雄株对遮荫胁迫的抗性较雌株强。本结论对移栽银杏幼苗起到一定的指导作用,可优先选用雄株。In our experiment,seedlings of male and female Ginkgo biloba L.individuals were exposed to shade treatment with 57% sun light comparing with ambient environment.It was carried out with two factors （sex and shade stress） layout.The results using ultraviolet spectrophotometry are as follows：（1） For both sex tested,shade resulted in lower needle superoxide dismutase（SOD）,female seedlings decreased 32.69% and male seedlings decreased 27.51%; （2） Shade resulted in lower needle peroxidase（POD）,female seedlings decreased 76.14% and male seedlings decreased 53.88%; （3） The contents of soluble protein of female and male seedlings decreased 12.02%,2.74%,respectively.（4） The malondialdehyde （MDA） content of female and male were increased 79.06%,51.34% in shade stress respectively.Female seedlings response more sensitive to shade.All of changes of index are larger than male seedlings.Therefore female seedlings have stronger resistance than male seedlings.This results would make use of directing to transplant Ginkgo biloba L.for preferring male seedlings.

关 键 词：银杏 雌雄异株 遮荫 抗氧化系统酶 生化物质 紫外分光光度法 

分 类 号：O657.32[理学—分析化学]