基于固相抗体模式时间分辨荧光免疫分析测定雌三醇  

Time-Resolved Fluoroimmunoassay for Estriol Based on Solid Phase Coated Antibody Competitive Mode

作　　者：高素芹[1] 杜凌云[1] 王术皓[1] 

机构地区：[1]聊城大学化学化工学院,山东省聊城市湖南路1号252059

出　　处：《光谱实验室》2010年第3期1089-1093,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(20877037)资助;山东省自然科学基金(2005ZX12);山东省"泰山学者"建设工程专项经费资助

摘　　要：采用二步标记法,借助二乙烯三胺五乙酸酐,将Eu3+标记到雌三醇完全抗原上,基于固相抗体竞争反应模式,建立了时间分辨荧光免疫分析测定雌三醇的新方法。在优化的条件下,方法的线性范围为0.005—1000ng·mL-1,检出限为4pg·mL-1。该方法用于检测血清中雌三醇的含量,结果令人满意。A rapid and sensitive method based on solid phase coated antibody by time-resolved fluoroimmunoassay for the determination of estriol was established.The estriol-ovalbumin （E3-OVA） was labeled with europium ion （Eu3＋） by means of a simple method involving the anhydride of diethylenetriam inepentaacetia acia（DTPAA）.Under the optimal conditions,the linear range of the method was from 0.005 to 1000ng·mL-1,and the limit of detection was found to be 4pg·mL-1.The method has been applied to determine estriol in serum sample with satisfactory results.

关 键 词：固相抗体包被 时间分辨荧光免疫分析 雌三醇 铕 

分 类 号：O657.39[理学—分析化学]