应用光镊子拉曼光谱鉴别阴道毛滴虫和口腔毛滴虫的研究  

Identification of Trichomonas Vaginalis and Trichomonas Tenax Using Laser Tweezers Raman Spectroscopy

作　　者：梁裕芬[1] 韦俊彬[1] 毛丽华[2,3] 黄庶识[3] 

机构地区：[1]广西中医学院基础医学院,南宁市明秀路179号530001 [2]广西师范大学物理科学与技术学院,广西桂林市541004 [3]广西科学院生物物理实验室,南宁市530003

出　　处：《光谱实验室》2010年第3期1143-1147,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：广西自然科学基金项目(No.0728177);广西中医学院自然科学研究基金(P2008099)资助

摘　　要：应用激光镊子拉曼光谱技术比较分析阴道毛滴虫和口腔毛滴虫的拉曼吸收峰,寻找具有分类学鉴别价值的特征拉曼峰。通过光镊随机俘获单个大小一致、有活力的虫体,记录其拉曼光谱,收集数据并做主成分分析。在937cm-1、1002cm-1和1446cm-1谱峰的强度和谱型上有差异,根据1002cm-1峰的信号强度,并结合937cm-1和1002cm-1峰强度比值(I937/I1002)以及1002cm-1和1446cm-1峰强度比值(I1002/I1446)可以作为区分阴道毛滴虫和口腔毛滴虫的量化指标。该法是简便、快捷、有效地鉴别两种人体毛滴虫的新方法。Laser tweezers Raman spectroscopy was used to identify Trichomonas vaginalis（TV） and Trichomonas tenax（TT）.The Raman spectra of 30 trichomonads were randomly acquired,in which 20 polypides were randomly used to carry out a principal components analysis（PCA）.The mean Raman spectra of TV among their strains were as well as TT,but the Raman bands at 1002cm-1 of TV showed significant differences compared to TT and the intensity of TV at 1002cm-1 peak is obviously higher around 2 folds than TT.The ratio I937/I1002 of TV strains was lower than those of TT strains,on the contrary,the ratio I1002/I1446 of TV strains was higher about 2 folds than TT strain＇s.Above values can be used as an index quantificationally to identify TV and TT.The method can be used to rapidly discriminate trichomonads from various species.

关 键 词：光镊子 拉曼光谱 阴道毛滴虫 口腔毛滴虫 

分 类 号：O657.37[理学—分析化学]