显微操作组织贴块法培养大鼠血管内皮细胞  

Cultivation of vascular endothelial cells of albino rat artery using tissue explants adhension and micrurgy method

作　　者：王定坤[1] 杜智勇[2] 徐在品[1] 王清宇[1] 赵伟[1] 蒋腾蛟[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]黔东南民族职业技术学院,贵州凯里556000

出　　处：《黑龙江畜牧兽医》2010年第5期11-13,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(10772054;10572029);贵州大学研究生创新基金项目(省研农2009001);贵州大学SRT项目(贵大SRT2009055)

摘　　要：为了探讨显微操作组织贴块法培养大鼠动脉血管内皮细胞的效果,试验用倒置相差显微镜观察细胞形态和生长特征,用免疫细胞化学法检验内皮细胞Ⅷ因子进行血管内皮细胞鉴定。结果表明:72h后内皮细胞从组织块边缘游出,12d后细胞开始融合成片,内皮细胞阳性率为97%。说明应用显微操作组织贴块法培养能获得高纯度血管内皮细胞。To explore effects of micrurgy and tissue explants adhension to culture vascular endothelial cells from albino rat aorta.The cell growth was evidenced by revealing the morphological characteristics under inverted phase contrast microscope and detection of Ⅷ factor（or von Willebrand factor,vWF）by immunocytochemistry.The results showed that the migration of endothelial cells from aortic cub was found after 72h＇s cultivation,forming confluent monolayer of a cobblestone pattern after 12 days.Cell positive rate of vWF in the cytoplasm of the new cells was 97%.Combination micrurgy with tissue explants adhension could obtain pure vascular endothelial cells.

关 键 词：显微操作 血管内皮细胞 原代培养 大鼠 

分 类 号：S852.13[农业科学—基础兽医学]