AGEs通过SDF-1/CXCR4轴信号通路对心肌微血管内皮细胞血管新生的影响及机制  

Effect of AGEs on microvascular endothelial-cells angiogenesis by SDF-1/CXCR4 axis signaling pathway

作　　者：粟妍晖[1] 李飞[1] 王冬娟[1] 刘楠[1] 王海昌[1] 

机构地区：[1]第四军医大学西京医院心血管内科,陕西西安710032

出　　处：《现代生物医学进展》2010年第7期1270-1272,共3页Progress in Modern Biomedicine

摘　　要：目的:探讨AGEs通过刺激SDF-1/CXCR4轴信号系统对心肌微血管内皮细胞的增值、迁移、管样结果形成的影响以及AMD3100对其的干预作用。方法:用不同浓度的AMD3100作用于浓度为200mg/L的AGEs共孵育的CMECs24h,用MTT法检测细胞活力及增殖能力,并选择合适的干扰浓度(抑制效果居中)。随机选取加入AGEs200ml/L的CMECs,加入合适浓度的AMD3100,分别作用24、48、72h,采用MTT法测定AGEs处理前后细胞增值率的变化,并检测AMD3100对增值率的影响;毛细血管管腔样结构形成实验检测对CMECs血管新生的影响和AMD3100对其阻断作用的影响。结果:心肌微血管内皮细胞增殖能力和迁移能力在24h、48h、72h有显著增强;并促进了心肌微血管内皮细胞管样形成(vsP<0.05);CXCR4受体阻断剂AMD3100作用于细胞后,可以显著阻断AGEs对心肌微血管内皮细胞增殖能力和迁移能力和管腔形成(vsP<0.05)的影响。结论:AGEs在早期显著增强了心肌微血管内皮细胞的增殖、迁移和管样结构形成的能力,其作用机制可能与SDF-1/CXCR4轴信号通路有关。Objective：To observe the effects of the advanced glycation end products（AGEs） on proliferation,migration,angiogenesis and lumen formation of cardiac microvascular endothelial cells（CMECs） by SDF-1/CXCR4 axis signaling pathway.Method：Add different concentrations of AMD3100 to CMECs incubated with AGEs 9200mg/L） for 24h.MTT were used to detect the cell viability and proliferation.Ac-cording to the results,chose a concentration for next experiment.AGEs with the CXCR4 blocker AMD3100 1ng/mL were added with CMECs of 24h、48h、72h,Then,proliferation and migration were detected by MTT and transwell.Capillary lumen formation test was applyed to detect the angiogenesis.Result：CMECs in the AGEs concentration of 200 mg/L after 24h、48h、72h incubation,the A values were 0.1067 ±0.0113;0.1422 ±0.0058;0.1855 ±0.0225,which significantly higher than 0.0883 ±0.0233;0.1075±0.0184;0.1397±0206（P0.01）;and tubule-like formation structure of CMECs were 3.416±0.791,4.737±1.129,7.470±1.890 higher than 2.227±0.6149,2.716±1.186,3.487±1.722）m/m2（P0.05 vs.control group）.All of those showed time-dependent.CONCLUSION：AGEs ad-vanced proliferation,migration and tubule-like structure formation with time-dependent and.can enhance the proliferation,migration,angiogenesis and lumen formation of cardiac microvascular endothelial cells（CMECs） by SDF-1/CXCR4 axis sinnaling pathway.Conclusion：AGEs were significantly increased in the early cardiac microvascular endothelial cell proliferation,migration and lumen forma tion capabilities,and its mechanism may be related to SDF-1/CXCR4 axis pathway.

关 键 词：糖基化终产物 基质细胞衍生因子-1 CXCR4 心肌微血管内皮细胞 管腔结构 

分 类 号：R543[医药卫生—心血管疾病] R587.1[医药卫生—内科学]