运动对SD大鼠脑组织MDA、SOD和NGF含量的影响  

The Effects of Exercises on Cerebral Function of SD Rats

作　　者：顾丽燕[1] 赵娜娜[1] 梁丹丹 

机构地区：[1]首都体育学院康复教研室,北京100088 [2]安徽省巢湖市职业技术学院康复医学教研室,238000

出　　处：《现代生物医学进展》2010年第7期1277-1279,共3页Progress in Modern Biomedicine

基　　金：北京市市属市管高校北京市重点实验室开放研究课题-近红外光谱技术在脑血管意外的康复中的运用的研究

摘　　要：目的:观察运动对脑功能的影响,为临床上运动疗法改善脑功能提供实验依据。方法:雄性SD大鼠24只,随机分为3组,分别为安静对照组(C组)8只,4周运动组(4E)8只,8周运动组(8E)8只,C组安静喂养,不进行运动训练,4E组适应性运动一周后进行4周跑台训练,8E组适应性训练一周后进行8周跑台训练,跑台坡度5°,每周运动5天。两组运动组分别在运动时间结束后取材,取大鼠脑组织,取右脑测定MDA、SOD、NGF含量。结果:经过4周跑台运动后,4E组MDA含量(3.75±0.63)nmol/mgprot,与C组(3.867±0.57)nmol/mgprot相比无明显差异,SOD含量(39.67±8.43)NU/mgprot,高于C组(30.14±9.60)NU/mgprot(P<0.05),NGF含量(2.13±0.37)pg/mgpro高于对照组(1.42±0.12)pg/mgpro(P<0.01);经过8周跑台运动后,8E组MDA含量(2.12±0.42)nmol/mgprot,低于C组(3.867±0.57)nmol/mgprot(P<0.05),且低于4E组(P<0.05),SOD含量(42.20±7.21)NU/mgprot,高于C组(30.14±9.60)NU/mgprot(P<0.01),NGF含量(2.58±0.43)pg/mgpro高于对照组(1.42±0.12)pg/mgpro(P<0.01),但与4E组间无明显差异。结论:长期有氧运动可降低大鼠脑组织中脂质过氧化水平,减少氧自由基的损害作用,同时提高NGF水平,促进脑组织再生修复能力。Objects：To provide evidence for using exercises therapy in clinic work,we observed the effect of exercises on cerebral function of SD rats.Methods：We divided 24 male SD rats into three groups：control group（C group）,4 weeks exercises group（4E group）,8 weeks exercises group（8E group）.After 0me-day adaptive exercise,4E/8E group began 4w/8w treadmill exercises training for 5 days a week.The grade of treadmill was 5°.After exercises training,we cut the heads of SD rats and got brain tissue for MDA.SOD.NGF determine.Results：The content of MDA of 4E group were 3.75±0.63 nmol/mgprot,no clear deferens with C group,SOD were 39.67±8.43 mgprot,and NGF were 2.13±0.37 pg/mgpro,both higher than C group（P0.01）.The content of MDA of 8E group were 2.12±0.42 nmol/mgprot,lower than C group and 4E group（P0.05）,SOD were 42.20 ±7.21 NU/mgprot,NGF were 2.58±0.43 pg/mgpro,both higher than C group.（P0.01）.Conclusion：The level of lipid peroxidation would be depressed by long-term aerobic exercise,which depressed the damage of oxygen free radical,and exercise could elevate the level of NGF,improving the recovery and restoration of brain.

关 键 词：跑台运动 大鼠 脑MDA SOD NGF 

分 类 号：Q95-3[生物学—动物学] R87[医药卫生—运动医学]