TUNEL法探讨UCP2敲除对糖尿病心肌细胞凋亡的影响  

The effect on myocardial apoptosis of diabetic mellitus of UCP2 in TUNEL

作　　者：李秀珍[1] 谭若芸[2] 鲁翔[1] 

机构地区：[1]南京医科大学第一临床医学院心内科,江苏南京210029 [2]南京医科大学第二临床医学院肾内科,江苏南京210011

出　　处：《现代生物医学进展》2010年第7期1346-1347,1359,共3页Progress in Modern Biomedicine

基　　金：江苏省科技厅社会发展基金(BS2006007)

摘　　要：目的:探讨UCP2对糖尿病小鼠心肌细胞的凋亡作用。方法:建立解耦联蛋白2(Uncoupling protein2,UCP2)敲除(UCP2-/-)的糖尿病模型,用原位脱氧糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测,显微成像系统采集图像,经计算机图像系统分析及统计学处理。结果:TUNEL阳性信号为棕色颗粒,即为细胞凋亡。结论:UCP2影响糖尿病心肌细胞凋亡。Objective：To explore the role of uncoupling protein 2 to myocardial apoptosis of diabetic mellitus;Methods：making the animal models of diabetic mellitus,in the TUNEL apoptotic detection in situ,in microscopy imaging system collecting the image data,the computer imagine processing system was adapted as well for the observation and analysis;Result：yellow or yellowish green fluorescence showed the stronger positive signal of cell apoptosis;Conclusion：UCP2 affected myocardial apoptosis of diabetic mellitus.

关 键 词：解耦联蛋白2 原位缺口末端标记法 凋亡 

分 类 号：R587.1[医药卫生—内分泌]