大熊猫Sjogren综合症/硬皮病自身抗原1基因(SSSCA1)的克隆及比较  

cDNA cloning and comparative analysis of Sjogren's syndrome/scleroderma autoantigen 1(SSSCA1) gene of the giant panda

作　　者：侯怡铃[1] 张田[1] 侯万儒[1] 

机构地区：[1]西华师范大学生命科学学院,南充637002

出　　处：《兽类学报》2010年第2期212-217,共6页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(30470261);四川省应用技术资助项目(2006J13-057);四川省教育厅重点科研项目(07ZA120);四川省重点学科动物学建设经费资助(404001);四川省应用基础项目(2009JY006)

摘　　要：Scleroderma is a connective tissue disease characterized by the fibrosisization of skin and internal organs.In this study,the genomic sequence and cDNA of Sjogren’s syndrome/scleroderma autoantigen 1(SSSCA1)gene of the giant panda was cloned successfully using RT-PCR.The cDNA fragment cloned was 642 bp in length,and contained an open reading frame (ORF) of 600 bp encoding 199 amino acids.The genomic sequence was 1 262 bp,containing four exons and three introns. The deduced amino acid sequence showed that this protein was composed of 199 amino acids and its estimated molecular weight was 21.53841 kDa with a pI of 5.14. Three different patterns of functional sites were found:one protein kinase C phosphorylation site,five casein kinase Ⅱ phosphorylation sites,and three N-myristoylation sites. The SSSCA1 gene of the giant panda were highly homologous to those of some other mammals.Scleroderma is a connective tissue disease characterized by the fibrosisization of skin and internal organs.In this study,the genomic sequence and cDNA of Sjogren＇s syndrome/scleroderma autoantigen 1（SSSCA1）gene of the giant panda was cloned successfully using RT-PCR.The cDNA fragment cloned was 642 bp in length,and contained an open reading frame （ORF） of 600 bp encoding 199 amino acids.The genomic sequence was 1 262 bp,containing four exons and three introns. The deduced amino acid sequence showed that this protein was composed of 199 amino acids and its estimated molecular weight was 21.53841 kDa with a pI of 5.14. Three different patterns of functional sites were found：one protein kinase C phosphorylation site,five casein kinase Ⅱ phosphorylation sites,and three N-myristoylation sites. The SSSCA1 gene of the giant panda were highly homologous to those of some other mammals.

关 键 词：大熊猫 RT-PCR SSSCA1 克隆 

分 类 号：Q751[生物学—分子生物学]