T细胞受体Vβ亚家族基因表达特点与特发性血小板减少性紫癜临床疗效的相关性  

T Cell receptor variable region β chain subfamily gene expression characteristics correlate with clinical therapy and effects of Idiopathic Thrombocytopenic Purpura

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作  者:范建新[1] 

机构地区:[1]山西医科大学第二医院血液科,山西太原030001

出  处:《中国中医药咨讯》2010年第9期80-82,共3页

摘  要:目的研究特发性血小板减少性紫癫(ITP)患者T细胞受体p链可变区互补决定区3(TCRβVCDR3)基因表达的变化,与ITP临床治疗效果之间的关系。方法采用RT—PCR方法分析15例缓解组ITP患者、10例复发难治组ITP患者和6例正常对照组T细胞受体β链(TCRβ)的可变区(V)的24个家族的互补决定区3(CDR3)基因序列。结果15例缓解组ITP患者见部分CDR3基因谱型异常,平均每个患者基因异常的家族数为2.55±0.38个,未见T细胞克隆增生。10例复发难治组ITP患者中,在8V8、BV13.1、BV14和BV17等亚家族都出现了克隆性增生,平均每个患者异常家族为7.1±3.15个,明显高于正常对照组,差异有统计学意义(P〈0.05)。结论ITP的发病及预后与T细胞免疫状态有关,TCRBVCRD3基因谱型分析可以发现异常增生克隆,有助于判断ITP的治疗效果和预后。Objective: To study the T lymphocytic clones that correlate with clinical therapy and effects of idiopathic thrombocy- topenic purpura (ITP) by investigating gene expression changes of complementarity determining region (CDR3) repertoires of T cell receptors (TCRs) βchain variable region( β V). Methods: Reverse transcription polymerase chain reaction (RT-PCR) and gel electrophoresis was used to analyze 24 subfamily genes of TCR β V from peripheral blood lymphocytes (PBLs) of 15 patients with, 10 patients with refractory ITP and 6 normal control. SPSS 17.0 software was used to perform the statistical analysis. Results:In clinically lenitive rrP group, oligoclonality could be observed in 15 cases with an average value of 2.55 ±0.38 per person, abnormal CDR3 size distribution had little difference compared with normal controls (P〉0.05). Abnormal CDR3 distribution could be observed apparently different between refractory ITP patients and healthy controls (P〈0.001), with oligoclonality at an average value of 7.1 ±3.15 per person. In V β 8 ,V β 13.1 ,V β14 and V β17 subfamily, common clonal T cell expansions could be observed.Conclusion Clinically lenitive ITP group and refractory ITP group have different relations with T ceils related immunity. TCR β V CDR3 size distribution can help find abnormal T cell clones, which may contribute to the diagnosis, discrimination and thera[r,y of different types of ITP.

关 键 词:特发性血小板减少性紫癜 T细胞受体TCR β链V区 互补决定区3 

分 类 号:R554.6[医药卫生—血液循环系统疾病]

 

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