白屈菜药材的UPLC特征图谱建立及生物碱类成分的QTOF-MS分析  

Establishment the UPLC specific chromatogram of Chelidonium majus L. and its alkaloids compositions analysis by QTOF-MS

作　　者：顾悦[1] 钱大玮[2] 段金廒[2] 王振中[3] 孙国玲[1] 倪书茂[2] 郭建明[2] 

机构地区：[1]江苏大学药学院,镇江212013 [2]南京中医药大学,南京210046 [3]江苏康缘药业股份有限公司,连云港222001

出　　处：《药物分析杂志》2010年第5期780-786,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立白屈菜药材的UPLC-UV特征图谱并用QTOF-MS技术分析其生物碱类成分。方法:采用ACQUITY UPLC BEH C18(2.1mm×100mm,1.7μm)色谱柱,以乙腈-10mmol.L-1醋酸铵缓冲液(用醋酸调节至pH3.0)为流动相梯度洗脱,流速0.4mL.min-1,检测波长280nm,柱温35℃,对10批白屈菜药材的特征图谱进行比较研究,用UPLC-ESI-QTOF-MS技术对其共有峰进行分析。结果:获得白屈菜药材的UPLC特征图谱,共有峰17个,鉴定出14个生物碱成分,其中6个通过对照品对照确认,8个通过质谱信息推测。结论:建立的UPLC-UV特征图谱可用于白屈菜质量评价。Objective：To establish the UPLC specific chromatogram of Chelidonium majus L.and analyze the alkaloids compositions by QTOF-MS.Methods：The samples were separated by ACQUITY UPLC BEH C18 column（2.1 mm×100 mm,1.7 μm）and eluted with acetonitrile-10 mmol·L-1 ammonium acetate buffer（adjusted to pH 3.0 with acetic acid）at a flow rate of 0.4 mL·min-1.The detection wavelength was 280 nm and column temperature was 35 ℃.In the condition,10 samples of Chelidonium majus were analyzed.Common peaks were analyzed by UPLC-ESI-QTOF-MS.Results：The specific chromatograms of Chelidonium majus L.were obtained.There were 17 common peaks.Fourteen alkaloids compositions were identified.Among them,6 compositions were identified by direct comparison with the reference substances and others were identified by MS and MS2 data.Conclusion：UPLC specific chromatogram facilitates the evaluation of the quality of Chelidonium majus L..

关 键 词：白屈菜 UPLC 特征图谱 生物碱 QTOF-MS分析 

分 类 号：R917[医药卫生—药物分析学]